Selectable marker for development of vectors and transformation systems in plants
AssigneeEli Lilly and Company
InventorClive Waldron
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Abstract
The present invention discloses functional and selectable micro-Ti plasmids. The hygromycin phosphotransferase (aphIV) gene from Escherichia coli was inserted between the 5' promoter and associated amino terminal region-encoding sequence of an octopine synthetase gene and the 3' terminator signal sequence of a nopaline synthetase gene. These constructs were assembled between T-DNA border fragments in a broad-host-range vector and used to create antibiotic-resistant plant cells.
Claims27
A chimeric gene functional in a plant cell, which chimeric gene comprises:
- (a) at a position 5' to coding region (c), a plant-expressible promoter sequence;
- (b) at a position 3' to coding region (c), a terminator signal sequence;
- and
- (c) a coding region of an aphIV gene, which coding region:
- (i) encodes a functional hygromycin phosphotransferase enzyme;
- and (ii) is positioned between such plant-expressible promoter sequence (a) and such terminator signal sequence (b) so as to be expressible,
- wherein expression of such coding region in a plant cell confers resistance to hygromycin B on such plant cell and wherein such resistance to hygromycin B is capable of providing a basis for selection of such plant cell.
Plasmid pCEL40 shown in FIG. 2 and derivable by ligating the BglII digest of plasmid pCEL30, to the .about.1.3 kb BamHI-BglII fragment of plasmid pOW20, such plasmids pCEL30 and pOW20 being obtainable from NRRL B-15915 and NRRL B-15838, respectively.
A plant part from the plant of claim.
Dependencies are inferred from the claim text.