Passivated polymer supports for nucleic acid synthesis
AssigneeHybridon, Inc.
InventorsJin-Yan Tang, Jimmy X. Tang
Life of this patent
Estimating the term…
Abstract
The invention provides passivated organic polymer supports, processes for their preparation and processes for their use in oligonucleotide synthesis that allow for highly efficient solid phase synthesis of oligonucleotides.
Claims8
An organic polymer support for solid phase synthesis of oligonucleotides, such support comprising a plurality of passivated organic polymer microscopic particles, wherein each particle has amino groups and/or hydroxyl groups covalently bound to the particle surface, wherein each particle further has nucleosides covalently bound to some of the amino and/or hydroxyl groups, and wherein at least some of the amino and/or hydroxyl groups that are not covalently bound to nucleosides are covalently bound to a hydrophobic passivating group, wherein the hydrophobic passivating group is an aroyl group.
A process for passivating an organic polymer support for oligonucleotide synthesis, such progress comprising contacting an organic polymer support particle having a surface that has covalently bound free amino and/or hydroxyl groups and nucleosides covalently bound to the support via said amino and/or hydroxyl groups with a hydrophobic passivating reagent, wherein the passivating reagent is an acid anhydride of an aroyl group or an aroyl chloride.
Dependencies are inferred from the claim text.