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US 5,668,264 AGranted
High affinity PDGF nucleic acid ligands
AssigneeNeXstar Pharmaceuticals, Inc.
InventorsNebojsa Janjic, Larry Gold
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Abstract
Methods are described for the identification and preparation of high-affinity nucleic acid ligands to PDGF. Included in the invention are specific ssDNA and RNA ligands to PDGF identified by the SELEX method.
Claims7
A purified and isolated non-naturally occurring nucleic acid ligand to PDGF.
A nucleic acid ligand to Platelet Derived Growth Factor (PDGF) identified according to the method comprising:
- a) contacting a candidate mixture of nucleic acids with PDGF, wherein nucleic acids having an increased affinity to PDGF relative to the candidate mixture may be partitioned from the remainder of the candidate mixture;
- b) partitioning the increased affinity nucleic acids from the remainder of the candidate mixture;
- and
- c) amplifying the increased affinity nucleic acids to yield a mixture of nucleic acids enriched for nucleic acid sequences with relatively higher affinity and specificity for binding to PDGF, whereby a nucleic acid ligand of PDGF may be identified.
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Dependencies are inferred from the claim text.