Method of preparing and activating samples for radioimmunoassay and other test
OwnerJose Alberto Fernandez-Polinventor
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Abstract
A method of activating target proteins in a biologic sample for radioimmunoassay or other immunoassay by adding the sample to a diluent containing no calcium or magnesium, heating the mixture in a boiling water bath for a designated period of time, cooling the sample, centrifuging the mixture to isolate the activated protein containing supernatant, and then vortexing the supernatant to ensure homogeneity of the protein.
Claims17
A method of activating a protein in a sample for diagnostic procedures comprising the steps of:
- diluting a predetermined quantity of the sample with a predetermined quantity of a diluent, said diluent having no calcium and magnesium;
- mixing the serum and diluent;
- heating the serum and diluent to 100.degree. C. for a predetermined time period;
- cooling the serum and diluent;
- centrifuging the serum and diluent;
- removing a supernatant liquid;
- and
- vortexing the remaining material;
- wherein the predetermined period of time for heating the sample and diluent is approximately 3 to 5 minutes;
- wherein the diluent is a phosphate buffer saline solution without calcium and magnesium.
A method of releasing bound target proteins in a sample for radioimmunoassay testing comprising the steps of:
- diluting a predetermined quantity of sample with a predetermined quantity of a phosphate-buffered saline diluent, said diluent having no calcium and magnesium, to form a in a mixture of said sample in said diluent;
- heating the mixture in a boiling water bath to 100.degree. C. for a predetermined amount of time;
- centrifuging the mixture;
- and
- removing a protein-containing supernatants;
- wherein the predetermined period of time for heating the sample and diluent is approximately 3 to 5 minutes;
- wherein the diluent is a phosphate buffer saline solution without calcium and magnesium.
A method of activating and releasing target proteins in a biologic fluid for radioimmunoassay comprising the steps of:
- adding about 200 .mu.L of biologic fluid to about 400 .mu.L of buffered saline solution to form a biologic fluid in buffered saline solution mixture;
- heating said mixture boiling water bath to 100.degree. C. for approximately three minutes;
- cooling the mixture at room temperature;
- centrifuging the mixture at approximately 14,000.times. g at about 2 to 4 degrees C. for approximately twenty minutes;
- removing the activated protein containing supernatants from the centrifuged mixture;
- and
- vortexing the supernatants to ensure homogeneity of the activated proteins;
- wherein the diluent is a phosphate buffer saline solution without calcium and magnesium.
A method of activating by unbinding target proteins in a biologic fluid sample from non-target proteins for analysis of the target proteins comprising the steps of:
- adding a predetermined amount of serum containing the target proteins bound to non-target proteins to a physiologically buffered saline solution, said buffered saline solution having no magnesium and calcium;
- and
- heating the serum and diluent at approximately 3 to five minutes so as to activate the target proteins by unbinding the target proteins from non-target proteins;
- wherein the diluent is a phosphate buffer saline solution without calcium and magnesium.
Dependencies are inferred from the claim text.