US 5,668,007 A · Recombinant 21 kD cocoa protein and precursor

Abstract

A 21 kD protein, and its 23 kD expression precursor, as the source of peptide flavor precursors in cocoa (Theobroma cacao) have been identified. Genes coding for them have been probed, identified and sequenced, and recombinant proteins have been synthesized.

Claims13

Dependent claims

A purified and isolated 23 kD protein of Theobroma cacao, having the amino acid sequence shown in FIG. 2 (SEQ ID NO:

Dependencies are inferred from the claim text.

Facts

Application

07/949,812Filed 11 Dec 1992

Priority

GB 901301711 Jun 1990

Granted

16 Sep 1997

Term ends

Not estimatedThe source records don’t support an estimate.

Classification12 codes

A23G 1/423
Containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins: containing microorganisms or enzymes
A23G 1/02
Cocoa; Cocoa products, e.g. chocolate; Substitutes therefor: preliminary treatment, e.g. fermentation of cocoa
C07K 14/415
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from plants
C12N 15/73
Vectors or expression systems specially adapted for E. coli: expression systems using phage regulatory sequences

Recombinant 21 kD cocoa protein and precursor

Life of this patent

Abstract

Claims13

Recombinant 21 kD cocoa protein and precursor

Life of this patent

Abstract

Claims13