Cloned genes encoding reverse transcriptase lacking RNASE H activity
AssigneeLife Technologies Corporation
InventorsMichael Leslie Kotewicz, Gary Floyd Gerard
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Abstract
The invention relates to a gene which encodes reverse transcriptase having DNA polymerase activity and substantially no RNase H activity. The invention also relates to vectors containing the gene and hosts transformed with the vectors of the invention. The invention also relates to a method of producing reverse transcriptase having DNA polymerase activity and substantially no RNase H activity by expressing the reverse transcriptase genes of the present invention in a host. The invention also relates to a method of producing cDNA from mRNA using the reverse transcriptase of the invention. The invention also relates to a kit for the preparation of cDNA from mRNA comprising the reverse transcriptase of the invention.
Claims37
An isolated DNA molecule comprising a nucleotide sequence encoding a polypeptide having DNA polymerase activity and substantially no RNaseH activity, wherein said nucleotide sequence is derived from a Moloney murine leukemia virus (M-MLV) nucleotide sequence.
A method for preparing a DNA molecule, said method comprising
- (a) mixing an mRNA template with a polypeptide having DNA polymerase activity and substantially no RNaseH activity, wherein said polypeptide is encoded by a nucleotide sequence derived from a Moloney murine leukemia virus (M-MLV) nucleotide sequence;
- and
- (b) incubating said mixture under conditions sufficient to make a first DNA molecule complementary to said mRNA template.
A polypeptide having DNA polymerase activity and substantially no RNaseH activity, wherein said polypeptide is encoded by a nucleotide sequence derived from a Moloney murine leukemia virus (M-MLV) nucleotide sequence.
Dependencies are inferred from the claim text.