3-hydroxy-3-methyl-glutaryl-coa synthase preparation with improved stability
AssigneeMCW Research Foundation, Inc.
InventorHenry M. Miziorko
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Abstract
A preparation of recombinant 3-hydroxy-3-methylglutaryl-CoA synthase is disclosed, wherein the preparation has at least 0.024 unitsg specific activity. Preferably, the preparation has at least 0.24 units/mg specific activity and is a crude cell extract. Preferably, at least 90% of the synthase molecules have not been substantially proteolytically cleaved. A preparation of recombinant HMG-CoA synthase wherein the preparation retains 50% activity after storage at 4.degree. C. for six months is also disclosed. A method of evaluating the efficacy of candidate anti-isoprene and anti-cholesterol drugs is also disclosed which comprises exposing the candidate drug to a recombinant 3-hydroxy-3-methylglutaryl CoA synthase preparation.
Claims5
A preparation of recombinant avian cytosolic 3-hydroxy-3-methylglutaryl-CoA synthase, wherein the synthase is recombinantly produced in a transformed E. Coli BL21(DE3) host cell by the inducible transcriptional activity of a bacteriophage T7 RNA polymerase, wherein the preparation has at least 0.24 units/mg specific activity, wherein at least 90% of the synthase molecules have not been substantially proteolytically cleaved, and wherein the preparation retains 50% activity after storage at 4.degree. C. for six months.
Dependencies are inferred from the claim text.