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US 5,668,000 AGranted
Mitochondrial P450
AssigneeSumitomo Chemical Company, Limited
InventorsMegumi Akiyoshi, Yoshiyasu Yabusaki, Toshiyuki Sakaki, Hideo Ohkawa
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AssigneeSumitomo Chemical Company, Limited
InventorsMegumi Akiyoshi, Yoshiyasu Yabusaki, Toshiyuki Sakaki, Hideo Ohkawa
Estimating the term…
The expression of mitochondrial P450 in yeast is disclosed. The mitochondrial P450 includes a chimeric P450 in which a signal sequence present at the N-terminus of a mammalian mitochondrial P450 has been substituted by a signal of a yeast mitochondrial protein and a chimeric P450 in which the latter signal has been further substituted by a targeting signal sequence to microsome. An expression plasmid for producing the enzyme in a large scale, a recombinant yeast strain carrying the expression plasmid, a process for producing the enzyme by the use of the recombinant yeast, and a process for producing 5.beta.-cholestane-3.alpha., 7.alpha., 12.alpha., 27-tetrol, 25-hydroxyvitamin D.sub.3 and 1.alpha.,25-dihydroxyvitamin D.sub.3 are also disclosed.
A yeast expression plasmid comprising DNA encoding a mitochondrial P450 which is a chimeric cytochrome P450.sub.17.alpha.-c25 which consists of P450.sub.c25 having its targeting signal substituted by DNA encoding the N-terminal 15 amino acids of bovine adrenal cytochrome P450.sub.17.alpha., operably linked to a promoter and terminator that is operative in yeast.
The yeast expression plasmid pAMS25.
The yeast expression plasmid pRXMS25.
Dependencies are inferred from the claim text.