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US 5,667,997 AGranted
C8 and C10 medium-chain thioesterases in plants
AssigneeCalgene, Inc.
InventorsToni Alois Voelker, Huw Maelor Davies, Deborah Knutzon
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AssigneeCalgene, Inc.
InventorsToni Alois Voelker, Huw Maelor Davies, Deborah Knutzon
Estimating the term…
By this invention, further plant medium-chain acyl-ACP thioesterases are provided, as well as uses of long-chain thioesterase sequences in conjunction with medium-chain thioesterase sequences. In a first embodiment, this invention relates to particular medium-chain thioesterase sequences from elm and Cuphea, and to DNA constructs for the expression of these thioesterases in host cells for production of C8 and C10 fatty acids. Other aspects of this invention relate to methods for using plant medium-chain thioesterases or medium-chain thioesterases from non-plant sources to provide medium-chain fatty acids in plant cells. As a further aspect, uses of long-chain thioesterase sequences for anti-sense methods in plant cells in conjunction with expression of medium-chain thioesterases in plant cells is described.
A recombinant DNA construct comprising a plant medium-chain preferring acyl-ACP thioesterase encoding sequence, wherein said thioesterase has hydrolysis activity towards C8 and C10 fatty acyl-ACP substrates.
A recombinant DNA construct comprising an expression cassette capable of producing a plant medium-chain preferring acyl-ACP thioesterase in a host cell, wherein said construct comprises, in the 5' to 3' direction of transcription, a transcriptional initiation regulatory region functional in said host cell, a translational initiation regulatory region functional in said host cell, a DNA sequence encoding a biologically active plant medium-chain preferring acyl-ACP thioesterase having activity towards C8 and C10 fatty acyl-ACP substrates, and a transcriptional and translational termination regulatory region functional in said host cell, wherein said plant thioesterase encoding sequence is under the control of said regulatory regions.
A transgenic host cell comprising an expressed plant medium-chain preferring acyl-ACP thioesterase having activity towards C8 and C10 fatty acyl-ACP substrates.
A method of producing C8 and C10 fatty acids in a plant host cell, wherein said method comprises:
Dependencies are inferred from the claim text.