Process for production of bacterial cells containing poly-3-hydroxy butyric acid
AssigneeMitsubishi Gas Chemical Company, Inc.
InventorsShunichiro Minagawa, Shigeki Imagawa, Iwao Terao, Torakazu Tahara
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Abstract
Disclosed is a process for accumulating a poly-3-hydroxy butyric acid in bacterial cells by continuously fermenting a methanol-assimilating bacterium having a capability of producing a poly-3-hydroxy butyric acid, in a single fermentation vessel by using methanol as carbon source at a limited feeding rate of nitrogen, phosphorus, or potassium such that the retention time for the fermentation is more than 10 hours.
Claims4
A single stage continuous fermentation process for the production of bacterial cells containing a poly-3-hydroxy burytic acid comprising producing said bacterial cells under the following conditions:
- a) using as said bacterial cells a methanol-assimilating bacterium selected from the group consisting of Protomonas extorquens K (FERM BP 3548), Hyphomicrobium methylovorum (IFO 14180), Hyphomicrobium hollandism (IFO 21611 ), Paracoccus dentrificans (DSM I403), and mutants thereof having the ability to produce poly-3-hydroxy butyric acid under the continuous fermentation process conditions herein set forth;
- b) using methanol as a source of carbon;
- and
- c) undertaking the continuous fermentation at a retention time of more than I 0 hours by limiting the provision of a source of nitrogen, phosphorous, or potassium.
Dependencies are inferred from the claim text.