Aspergillus expression system
AssigneeNovo Nordisk Biotech, Inc.
InventorsRandy Michael Berka, Wendy Yoder, Shinobu Takagi, Karuppan Chettier Boominathan
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Abstract
The present invention relates to a novel expression system in which A. japonicus-type species are used as host cells for expression of heterologous proteins.
Claims28
An Aspergillus japonicus-type host cell comprising a nucleic acid sequence encoding a heterologous protein operably linked to a promoter.
An Aspergillus japonicus-type host cell comprising a nucleic acid sequence encoding a heterologous fungal enzyme operably linked to a fungal promoter, and a selectable marker.
A method for producing a protein of interest which comprises culturing an Aspergillus japonicus-type host cell comprising a nucleic acid sequence encoding a heterologous protein operably linked to a promoter, under conditions which permit expression of the protein, and recovering the protein from culture.
An Aspergillus japonicus-type host cell comprising a recombinant nucleic acid sequence encoding a homologous protein operably linked to a promoter.
A method for producing a protein of interest which comprises culturing an Aspergillus japonicus-type host cell comprising a recombinant nucleic acid sequence encoding a homologous protein operably linked to a promoter, under conditions which permit expression of the protein, and recovering the protein from culture.
Dependencies are inferred from the claim text.