Yeast promoter for expressing heterologous polypeptides
AssigneeDelta Biotechnology Limited
InventorsAndrew R. Goodey, Darrell Sleep, Dina Vakeria
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Abstract
A yeast promoter comprising the promoter of the (cytoplasmically located glycerol-3-phosphate dehydrogenase gene (GPD 1) possesses advantageous capacity to regulate the expression of heterologous polypeptides in yeasts transformed therewith. Expression of such polypeptides utilizing the GPD1 promoter can be regulated by the presence(repressed) or absence(derepressed) of high levels of sucrose or glucose in the fermentation medium. Alternatively, a non-repressing carbon source, such as glycerol or ethanol, can be added to the fermentation medium.
Claims12
An isolated or synthetic promoter selected from the group consisting of the nucleic acid set forth as SEQ ID NO:
- 1 and a variant thereof, which variant:
- (i) is at least 100 nucleotides long;
- (ii) has at least 80% sequence identity with a fragment of SEQ ID NO:
- 1 which has the same length as the variant;
- (iii) retains at least 10% of the transcription rate capacity of the nucleic acid set forth as SEQ ID NO:
- 1;
- and
- (iv) is repressed by sucrose or glucose and derepressed by the absence of sucrose or glucose.
Dependencies are inferred from the claim text.