Sequencing of oligosaccharides
AssigneeOxford GlycoSystems Limited
InventorsRajesh B. Parekh, Sally B. Prime
Life of this patent
Estimating the term…
Abstract
A process suitable for use in the sequencing of an oligosaccharide compound includes a first analysis of a primary oligosaccharide compound's monosaccharide composition, selection of sequencing agents to apply to the oligosaccharide compound, applying a selected sequencing agent to the oligosaccharide compound, and analyzing a released monosaccharide to select a second sequencing agent. The sequencing agent may be, for example, an enzyme. The oligosaccharide compound may be, for example, an oligosaccharide, or a product of an oligosaccharide, or a species having an oligosaccharide portion.
Claims11
A process for sequencing a primary oligosaccharide compound which is an oligosaccharide or has an oligosaccharide portion, which comprises:
- (a) making a first analysis to determine a monosaccharide composition of said primary oligosaccharide compound;
- and
- (b) selecting at least one first sequencing agent based on said first analysis;
- and then
- (c) applying said at least one first selected sequencing agent to said primary oligosaccharide compound, to give products comprising a residual oligosaccharide compound and at least one monosaccharide released by said at least one first selected sequencing agent;
- (d) making a second analysis of said at least one monosaccharide in relation to said monosaccharide composition and thereby selecting, based on said second analysis, at least one second sequencing agent for reaction with said residual oligosaccharide compound.
Dependencies are inferred from the claim text.