Diagnostics and treatments for cancers expressing tyrosine phosphorylated CRKL protein
AssigneeChildren's Hospital Los Angeles
InventorsJohn H. Groffen, Nora C. Heisterkamp, Johanna Ten Hoeve
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Abstract
The invention relates to methods and kits for diagnosing cancers arising from cells which express tyrosine phosphorylated CRKL protein, such as cells having the Philadelphia (Ph) chromosome, which includes chronic myelogenous leukemia (CML) and acute lymphoblastic leukemia (ALL), through the detection of increased levels of phosphorylated CRKL protein or through the detection of increased CRKL gene copy or mRNA expression. The invention also relates to methods of treating such cancers.
Claims20
A method of diagnosing cancers arising from cells which express tyrosine phosphorylated CRKL protein, said method comprising
- a) obtaining a sample of cells suspected of expressing tyrosine phosphorylated CRKL protein;
- b) determining the percent increase in tyrosine phosphorylated CRKL protein relative to normal control cells wherein an increase in the expression of phosphorylated CRKL protein relative to controls is associated with the presence of cancer.
A kit for diagnosing cancers arising from cells which express tyrosine phosphorylated CRKL protein, said kit comprising
- a) reagent capable of specifically binding to tyrosine phosphorylated CRKL protein which reagent is capable of being detected directly or indirectly;
- b) instructions for using said reagent in an assay to detect the presence of tyrosine phosphorylated CRKL protein;
- c) buffer reagents and reaction vessels to accomplish said assay;
- and
- d) a control reagent comprising CRKL protein obtained from non-neoplastic cells and which control reagent is used as a reference for determining the percent increase of tyrosine phosphorylated CRKL protein.
A kit for diagnosing cancers arising from cells which express tyrosine phosphorylated CRKL protein, said kit comprising
- a) a reagent capable of specifically binding to non-phosphorylated CRKL protein and tyrosine phosphorylated CRKL protein, which reagent is capable of being detected directly or indirectly;
- b) optionally comprising an additional reagent which is capable of detecting only the phosphotyrosine residue on phosphorylated CRKL;
- and
- c) instructions for using said reagent or reagents in an assay to detect the presence of tyrosine phosphorylated CRKL protein.
Dependencies are inferred from the claim text.