Use of specific properties of allergens, allergens from animal or botanical sources and methods for their isolation
AssigneeLaboratorios Leti S.A.
InventorLubertus Berrens
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Abstract
It was found that atopic allergens have enzymatic properties, in particular the properties to hydrolyze amide and/or ester linkages. These properties may be used for various purposes, e.g. for analysis of samples, standardization of pharmaceutical compositions and also for the preparation of the allergens in a pure form.
Claims9
A method of hydrolyzing amide and/or ester linkages in a substrate, comprising:
- contacting a substantially purified form of an atopic allergen having protease properties with a substrate having amide and/or ester linkages to thereby hydrolyze said linkages, wherein said allergen is isolated from mammalian and non-mammalian excrement sources, and is capable of hydrolyzing proteins and split substrates specific for enzyme active sites.
Method of establishing the allergenic potency of environmental dust samples or individual allergenic preparations, comprising:
- preparing an aqueous extract of an environmental allergen or allergen preparation, and quantitatively measuring the protease activity of said aqueous extract towards different specific substrates, said substrates having amide and/or ester linkages.
A kit for establishing the allergenic presence of environmental dust samples or individual allergenic preparations, comprising:
- a carrier containing a substrate having amide and/or ester linkages, said carrier adapted to detect the protease activity of an aqueous extract of an environmental allergen, or of an allergenic preparation.
Dependencies are inferred from the claim text.