Method for determining DNA sequences

A method for sequencing DNA to ascertain the sequence of the four bases G, C, A, and T comprising the steps of:

• 1) producing a first set of reporter labelled DNA fragments for two of the four bases, each base having a different reporter;
• 2) producing a second set of reporter labelled DNA fragments for the remaining two of the four bases, each base having a different reporter;
• 3) separating the respective first and second sets by gel electrophoresis;
• 4) generating a first signal for each of the respective sets that varies in amplitude to form peaks in accordance with a characteristic of the reporter and the rate at which the labelled base moves through the gel;
• 5) generating a second signal for each of the respective sets that varies in amplitude to form peaks in accordance with a characteristic of the reporter and the rate at which the labelled base moves through the gel;
• 6) dividing each of the first and second sets into corresponding blocks of bases;
• 7) selecting the amount of shift between the corresponding blocks of the first and second sets which minimizes peak to peak variance, and;
• 8) interpolating the shifts between the corresponding blocks of the first and second sets to align the first and second sets into a single set of four signals corresponding to the four bases in the order of their movement through the gel.