T-cell receptor varible transcripts as disease related markers
AssigneeThe Board of Trustees of the Leland Stanford Junior University
InventorsLawrence Steinman, Jorge Oksenberg, Claude Bernard
Life of this patent
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Abstract
Methods are provided for determining relations between autoimmune degenerative diseases and specific variable regions of T-cell receptors as associated with the host HLA or T-cells associated with umbatting neoprofilerative diseases. By identifying the particular T-cell receptors which cause or are the disease in mammals, various prophylactic and therapeutic techniques may be employed for inhibiting the attack of the T-cell receptors on the native protein or tissue enhance the defense. In addition, individuals may be diagnosed as to their propensity for a particular autoimmune disease or the occurrence of such a disease.
Claims6
A method for determining the presence in a human host of T-cells associated with multiple sclerosis, said method comprising:
- isolating T-cells from the brain or cerebrospinal fluid of a human host;
- detecting in the T-cells of the brain or cerebrospinal fluid of said host the presence of a limited number of rearranged CDR3 regions of the T-cell receptor Valpha or Vbeta chain, wherein said rearrangement is associated with multiple sclerosis;
- wherein the presence of T cells associated with said multiple sclerosis is determined.
A method for determining the presence in a human host of T-cells associated with multiple sclerosis, said method comprising:
- isolating T-cells from the brain of a human host;
- detecting in the T-cells of the brain of said host the presence of a limited number of rearranged CDR3 regions of the T-cell receptor Valpha or Vbeta chain, wherein said rearrangement is associated with multiple sclerosis;
- wherein the presence of T cells associated with said multiple sclerosis is determined.
A method for determining the presence in a human host of T-cells associated with multiple sclerosis, said method comprising:
- isolating nucleic acid from T-cells from the brain of said human host;
- combining said nucleic acid with a first primer located 5' to a rearranged CDR3 and a second primer located 3' to the rearranged CDR3 of T-cell receptor Valpha or Vbeta chains, wherein said rearrangement is associated with multiple sclerosis;
- amplifying nucleic acid that hybridizes with said primers by means of the polymerase chain reaction;
- and
- determining the presence of a limited number of rearranged CDR3 regions, thereby determining the presence in a human host of T-cells associated with multiple sclerosis.
Dependencies are inferred from the claim text.