Skin substitute
AssigneeCentre International De Recherches Dermatologigues Galderma (Cird Galderma)
InventorsBruno Bernard, Marie-Cecile Lenoir, Braham Shroot, Yves-Michel Darmon, Daniel Asselineau
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Abstract
The invention concerns a skin substitute composed of a dermis equivalent covered with an epidermis equivalent. The dermis equivalent is a film formed from a type I collagen gel containing fibroblasts. The epidermis equivalent incorporates a basal membrane equivalent, basal layer cells, in a palisade arrangement attached to the membrane equivalent by hemidesmosomes, cells of the suprabasal layers, granular cells, and flat, keratinized cells arranged near the free surface.
Claims1
A skin substitute composed of a dermis equivalent covered with an epidermis equivalent, said dermis equivalent being a matrix formed from a type I collagen gel containing fibroblasts arranged three-dimensionally in said matrix, wherein said epidermis equivalent contains:
- (a) a basal membrane equivalent formed from the deposit of a layer of laminine, fibronectin and type IV collagen,
- (b) cells of the basal layer in a palisade arrangement, attached to the membrane equivalent according to paragraph (a) by hemidesmosomes and showing polar distribution of the bullous pemphigoid antigen;
- (c) cells of the suprabasal layers which, beginning with the first suprabasal layer, contain basic keratin with a value of 67 kDa, and acidic keratin with a value of 56.6 kDa;
- (d) granular cells containing involucrine, transglutaminase, filaggrine, keratohyaline granules and membrane coating granules, and
- (e) flat and keratinized cells arranged near the free surface, giving, after extraction using dodecyl sulfate and 2-mercaptoethanol, corneous envelopes characteristic of corneocytes, the cells of the different layers being attached with desmosomes.
Dependencies are inferred from the claim text.