Human recombinant collagen in the milk of transgenic animals
AssigneeCollagen Corporation
InventorRichard A. Berg
Life of this patent
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Abstract
Production of human procollagen or collagen in cells which ordinarily do not produce these molecules is effected by constructing expression systems compatible with mammary glands of non-human mammals. For example, expression systems can be microinjected into fertilized oocytes and reimplanted in foster mothers and carried to term in order to obtain transgenic non-human mammals capable of producing milk containing recombinant human procollagen or collagen. Human procollagen or collagen produced in this manner can be made of a single collagen type uncontaminated by other human or non-human collagens.
Claims2
Milk from a transgenic mouse, said mouse comprising a transgene which comprises a structural gene for a human .alpha.1(I) procollagen gene operably linked to a bovine .alpha.S1-casein promoter, said milk containing a recoverable amount of human trimeric .alpha.1(I) procollagen.
Milk containing a recoverable amount of a human trimeric .alpha.1(I) procollagen produced by recovering milk from the mammary glands of a transgenic mouse said mouse comprising a transgene which comprises a structural gene for said human .alpha.1(I) procollagen gene operably linked to a bovine .alpha.S1-casein promoter which effects expression of said structual gene specifically in milk protein-secreting epithelial cells of said mammary glands, wherein said human .alpha.1(I) procollagen is secreted into the milk of said mouse.
Dependencies are inferred from the claim text.