Vaccine for protection of cats against feline infectious peritonitis
AssigneePfizer Inc.
InventorsJay Dean Gerber, Jerald Dee Ingersoll
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Abstract
This invention relates to a temperature-sensitive (ts) feline infectious peritonitis (FIP) virus, which is characterized by substantially reduced growth at 39.degree. C. as compared with its growth at 31.degree. C. Also included in this invention is the use of the ts-FIP virus for oral or intranasal administration to cats as a vaccine to induce immunity against FIP.
Claims22
A method for protecting cats against infection by feline infectious peritonitis virus (FIP), which comprises intranasally administering an effective amount of an FIP Virus vaccine, said virus vaccine comprising a temperature-sensitive, attenuated FIP virus having a titer following propagation at 31.degree. C. which is at least about 1.times.10.sup.2 to about 1.times.10.sup.5 TCID.sub.50 greater than at 39.degree. C.
A device for immunizing cats against FIP infection, comprising a dropper or a spray bottle further comprising an FIP Virus vaccine, said virus vaccine comprising a temperature-sensitive, attenuated FIP Virus having a titer following propagation at 31.degree. C. which is at least about 1.times.10.sup.2 to about 1.times.10.sup.5 TCID.sub.50 greater than at 39.degree. C.
A feline infectious peritonitis (FIP) vaccine for oral or intranasal administration which, following administration, induces immunity against infection by FIP virus in cats without serious side effects, which comprises an effective amount of a temperature sensitive (ts), attenuated FIP virus, said virus having a titer following propagation at 31.degree. C. which is at least about 1.times.10.sup.2 to about 1.times.10.sup.5 TCID.sub.50 greater than at 39.degree. C.
A method of preparing a vaccine against FIP for oral or intranasal administration, which vaccine is capable of inducing immunity in cats against infection by FIP virus without serious side effects, comprising:
- a) culturing a virulent strain of FIP virus;
- b) attenuating the FIP virus of step (a) by high passage in susceptible cells;
- c) exposing the attenuated FIP virus of step (b) to a mutagenic agent to produce mutated, attenuated FIP viruses;
- d) selectively culturing from the mutated, attenuated FIP viruses of step (c) a temperature-sensitive, attenuated FIP virus having a titer following propagation at 31.degree. C. which is at least about 1.times.10.sup.2 to about 1.times.10.sup.5 TCID.sub.50 greater than at 39.degree. C.;
- and
- e) collecting the temperature-sensitive, attenuated FIP virus of step (d).
A vaccine dosage unit for inducing immunity in cats against infection by FIP virus, which comprises 0.5 to 1.5 ml of a liquid, suitable for intranasal or oral administration to a feline, said liquid containing 10.sup.2 to 10.sup.7 TCID.sub.50 of a temperature sensitive, attenuated FIP virus having a titer following propagation at 31.degree. C. which is at least about 1.times.10.sup.2 to about 1.times.10.sup.5 TCID.sub.50 greater than at 39.degree. C.
A kit comprising first and second components, the first component being a container further comprising an FIP vaccine in lyophilized form, said vaccine comprising an effective amount of a temperature-sensitive, attenuated FIP virus, said Virus having a titer following propagation at 31.degree. C. which is at least about 1.times.10.sup.2 to about 1.times.10.sup.5 TCID.sub.50 greater than at 39.degree. C., and the second component being a dropper suitable for intranasal administration comprising a diluent for reconstitution of the lyophilized vaccine.
Dependencies are inferred from the claim text.