Method and supports for gel permeation chromatography
AssigneeMerck Patent GmbH
InventorEgbert Muller
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Abstract
The invention relates to an improved method for gel permeation chromatography and to support materials for this method based on hydroxyl-containing base supports onto whose surfaces polymers are covalently bonded, wherein a) the base support comprises aliphatic hydroxyl groups, b) the covalently bonded polymers are bonded to the support via a terminal monomer unit, c) the linear polymers comprise monomer units of formula II, d) the polymers are crosslinked by ether bridges ##STR1## in which R.sup.1, R.sup.2 and R.sup.3 independently of one another are H or CH.sub.3, R.sup.4 is H or C.sub.1 -C.sub.5 -alkyl and n is an integer between 1 and 5.
Claims8
A support material for gel permeation chromatography, comprising a hydroxyl-containing base support on the surface of which polymers are covalently bonded, wherein:
- a) the base support comprises aliphatic hydroxyl groups,
- b) the covalently bonded polymers are bonded to the support via a terminal monomer unit,
- c) the polymer comprise monomer units of formula II, ##STR5## in which R.sup.1, R.sup.2 and R.sup.3 independently of one another are H or CH.sub.3
- R.sup.4 is H or C.sub.1 -C.sub.5 -alkyl and
- n is an integer between 1 and 5, and
- d) the polymers are crosslinked by ether bridges.
A process for the preparation of a support material comprising a hydroxyl-containing base support on the surface of which polymers are covalently bonded by graft polymerization, which process comprises polymerizing monomers of the formula I in solution in the presence of suspended particles of the hydroxyl-containing base support and cerium(IV) ions ##STR6## in which R.sup.1, R.sup.2 and R.sup.3 independently of one another are H or CH.sub.3,
- R.sup.4 is H or C.sub.1 -C.sub.5 -alkyl and
- n is an integer between 1 and 5;
- crosslinking the polymers by producing ether bridges therebetween;
- and converting the remaining epoxide groups to hydroxyl groups.
Dependencies are inferred from the claim text.