Sucrose phosphate synthase (SPS), its process for preparation its cDNA, and utilization of cDNA to modify the expression of SPS in plant cells
AssigneeCalgene, Inc.
InventorsCharles Van Assche, Danielle Lando, Jean Michel Bruneau, Toni Alois Voelker, Monica Gervais
Life of this patent
Estimating the term…
Abstract
Sucrose phosphate synthase (SPS), its process for preparation, its cDNA, and utilization of cDNA to modify the expression of SPS in the plant cells are provided.
Claims67
An isolated DNA encoding a sucrose phosphate synthase (SPS) derived from corn.
A recombinant construct comprising, as operably linked components in the 5' to 3' direction of transcription, a transcription initiation region functional in a vegetal cell and a DNA encoding a sucrose phosphate synthase (SPS) derived from corn.
A method of modifying the starch and sucrose levels in a tomato vegetal cell, said method comprising:
- growing a tomato vegetal cell having integrated into its genome a construct comprising, as operably linked components in the 5' to 3' direction of transcription, a transcription initiation region functional in said tomato vegetal cell and a DNA encoding a sucrose phosphate synthase derived from corn, wherein said DNA encoding said sucrose phosphate synthase derived from corn is not naturally linked to said transcription initiation region, wherein said tomato vegetal cell is grown under conditions which permit said transcription initiation region to function, and wherein growing said tomato vegetal cell under said conditions permits said DNA encoding said sucrose phosphate synthase derived from corn to be expressed at a level which modifies the starch and sucrose levels in said tomato vegetal cell from a given ratio of starch to sucrose, as measured in control plant cells, to a different ratio of starch to sucrose.
A tomato vegetal cell having integrated into its genome a recombinant construct of any one of claims 6-13, 60-61 and 64-65.
A tomato plant comprising cells having integrated into its genome a recombinant construct of any one of claims 6-13, 60-61 and 64-65.
A method of increasing the yield of a tomato plant comprising:
- growing a plant, wherein the genome of said plant comprises an integrated chimeric DNA construct capable of providing for expression of sucrose phosphate synthase derived from corn at a level sufficient to increase the amount of sucrose in tomato fruit by a factor of about 2 and decrease the amount of leaf starch by about 50% as compared to the amount of sucrose and starch measured in a control tomato plant, whereby an increase in plant yield is obtained.
An isolated DNA encoding a sucrose phosphate synthase wherein said DNA comprises at least about 10 nucleotides up to the full length of nucleotides represented by SEQ ID NO:
- 6.
A nucleic acid sequence encoding a peptide wherein said peptide has an amino acid sequence represented by a SEQ ID. NO:
- selected from the group consisting of SEQ ID. NO:
- 1, SEQ ID. NO:
- 2, SEQ ID. NO:
- 3, SEQ ID. NO:
- 4, and SEQ ID. NO:
- 5.
A method of increasing the yield of a tomato plant sink tissue, said method comprising:
- growing a tomato plant having integrated into its genome a construct comprising, as operably linked components in the 5' to 3' direction of transcription, a transcription initiation region functional in a tomato plant cell and a DNA encoding a sucrose phosphate synthase derived from corn, wherein said DNA encoding a sucrose phosphate synthase is not naturally linked to said transcription initiation region, and wherein said tomato plant cell is grown under conditions which permit said transcription initiation region to function,
- whereby the amount of sucrose available to said tomato plant sink tissue is increased compared to the amount of sucrose measured in a control tomato plant sink tissue.
A method of increasing the amount of soluble solids in a tomato plant sink tissue, said method comprising:
- growing a tomato plant having integrated into its genome a construct comprising, as operably linked components in the 5' to 3' direction of transcription, a transcription initiation region functional in a tomato plant cell and a DNA encoding a sucrose phosphate synthase derived from corn, wherein said DNA encoding a sucrose phosphate synthase is not naturally linked to said transcription initiation region, and wherein said tomato plant cell is grown under conditions which will permit said transcription initiation region to function,
- whereby the amount of soluble solids per unit weight of said tomato plant sink tissue is increased compared to the amount of solids measured in a control plant sink tissue;
- and
- whereby starch is converted to sucrose in said tomato plant cell and whereby an increased level of sucrose is made available to said tomato plant sink tissue.
Dependencies are inferred from the claim text.