Method for the rapid removal of protoporphyrin from protoporphyrin IX-containing solutions of hemoglobin
AssigneeSomatogen, Inc.
InventorsJames R. Ryland, Maura-Ann H. Matthews, Ulrich P. Ernst, Daniel E. Houk, David W. Traylor, Lee R. Williams
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Abstract
The present invention relates to a method for the production of a substantially protoporphyrin IX free hemoglobin solution comprising: rapidly heating a crude protoporphyrin IX-containing hemoglobin solution for a relatively short time and at a relatively high temperature to reduce protoporphyrin IX-containing hemoglobin to insignificant levels in said protoporphyrin IX-containing hemoglobin solution.
Claims38
A method for the production of a substantially protoporphyrin IX free hemoglobin solution comprising:
- heating a crude protoporphyrin IX-containing hemoglobin solution for a sufficient time of less than 5 minutes and at a sufficient temperature to reduce protoporphyrin IX-containing hemoglobin to insignificant levels to produce the substantially protoporphyrin IX free hemoglobin solution.
A method for the production of a substantially protoporphyrin IX free hemoglobin solution comprising:
- (a) exposing a crude protoporphyrin IX-containing hemoglobin solution to a non-oxygen gas, wherein said non-oxygen gas is selected from the group consisting of carbon monoxide and nitric oxide;
- and
- (b) heating the crude protoporphyrin IX-containing hemoglobin solution for a sufficient time of less than 5 minutes and at a sufficient temperature to reduce protoporphyrin IX-containing hemoglobin to insignificant levels to produce the substantially protoporphyrin IX free hemoglobin solution.
A method for the production of a substantially protoporphyrin IX free hemoglobin solution comprising:
- (a) exposing, via sparging, a crude protoporphyrin IX-containing hemoglobin solution to essentially pure carbon monoxide after breakage of cells containing a recombinant protoporphyrin IX-containing hemoglobin followed by
- (b) heating the crude protoporphyrin IX-containing hemoglobin solution via steam injection for from about 10 to about 11 seconds at about 81.degree. C. to reduce protoporphyrin IX-containing hemoglobin in said crude protoporphyrin IX-containing hemoglobin solution to less than about 1 percent of total hemoglobin, wherein said steam injection is accomplished with expansion steam injection.
Dependencies are inferred from the claim text.