Process for obtaining antibodies utilizing heat treatment
AssigneeCelltech Therapeutics Limited
InventorsAndrew Neil Charles Weir, Neil Andrew Bailey
Life of this patent
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Abstract
This invention relates to a process for obtaining antibodies in soluble and correctly folded and assembled form. It comprises a step to raise the temperature at a time in the process selected to facilitate the subsequent isolation of soluble, correctly folded and assembled antibody, substantially free of other antibody-related material. The operating temperature may be raised at any stage in the microbial fermentation or eukaryotic cell culture, or at any stage during extraction and purification of the antibodies.
Claims12
A process to facilitate the isolation of soluble, correctly-folded antibody molecules comprising subjecting a preparation comprising soluble, correctly-folded antibody molecules and partially degraded or incorrectly folded antibodies to a step to raise the operating temperature to an elevated temperature within the range of 34.degree. C. to 60.degree. C. in order to facilitate the removal of the partially degraded or incorrectly folded antibodies.
Dependencies are inferred from the claim text.