Pichia pastoris linear plasmids and DNA fragments thereof
AssigneeResearch Corporation Technologies, Inc.
InventorsMary Jane Hagenson, Kathryn A. Barr, David W. Stroman, Frank H. Gaertner, Michael M. Harpold, Ronald D. Klein
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Abstract
Two novel linear DNA plasmids are described. Also, novel fragments of the plasmids containing the autonomous replication sequence (ARS), and thus capable of self-maintenance as extra chromosomal elements are provided. These novel DNA sequences of the present invention are useful in a vector for self-maintenance in Pichia pastoris and are also useful as plasmid vectors which can be shuttled between yeast and bacteria.
Claims3
An isolated linear DNA plasmid obtainable from Pichia pastoris NRRL Y-11430 and having an approximate length of 14,000 base pairs and the restriction map of FIG. 1.
An isolated linear DNA plasmid obtainable from Pichia pastoris NRRL Y-11430 and having an approximate length of 10,000 base pairs and the restriction map of FIG. 2.
Dependencies are inferred from the claim text.