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US 5,665,590 AGranted
Method for isolating and directly cloning genes which encode cell-surface and secreted proteins
AssigneeSystemix, Inc.
InventorZhi Yang
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Abstract
Methods and DNA cloning vectors are provided for obtaining genes encoding secreted proteins and cell surface proteins.
Claims43
A composition comprising a DNA vector comprising, 5' to 3':
- a) a cloning site
- b) a region encoding an identifiable peptide;
- and
- c) a region encoding a membrane anchoring domain;
- wherein the identifiable peptide may also be the membrane anchoring domain, further wherein, upon cloning of a nucleotide encoding a signal peptide, or a functional portion thereof, in-frame with the region encoding the identifiable peptide, expression thereof results in appearance of the identifiable peptide on a cell surface.
2 more elements
Dependencies are inferred from the claim text.