Human liver epithelial cell lines
AssigneeThe United States of America as represented by the Department of Health and Human Services
InventorsCurtis C. Harris, Katharine H. Cole, John F. Lechner, Roger Reddel
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Abstract
Immortalized cell lines derived from normal adult human liver are described which express phenotypic characteristics of normal adult liver epithelial cells.
Claims15
A continuous epithelial cell line isolated from normal adult human liver tissue consisting of cells which have the following characteristics:
- 1) the cells are immortalized by transformation with a virus selected from the group consisting of SV40 virus, adenovirus, and papilloma virus;
- 2) the cells metabolically activate carcinogen precursor compounds to DNA-adduct forming compounds;
- 3) the cells demonstrate a pattern of gene expression, wherein said pattern of gene expression is such that the cells:
- a) do not express .alpha.-fetoprotein;
- and
- b) express mRNA encoding at least one of the proteins selected from the group consisting of a transferrin, .alpha.-1-antitrypsin, .alpha.2 macroglobulin, a catalase, superoxide dismutase and glutathione peroxidase;
- and
- 4) the cells are non-tumorigenic.
A continuous human liver epithelial cell line isolated from normal adult human liver tissue consisting of cells having the following characteristics:
- 1) the cells have been immortalized by transformation with SV40 T antigen;
- 2) the cells metabolically activate carcinogen precursor compounds to DNA-adduct forming compounds;
- 3) the cells demonstrate a pattern of gene expression, wherein said pattern of gene expression is such that the cells:
- a) do not express .alpha.-fetoprotein;
- and
- b) express mRNA encoding at least one of the proteins selected from the group consisting of a transferrin, .alpha.-1-antitrypsin, .alpha.2 macroglobulin, a catalase, superoxide dismutase and glutathione peroxidase;
- and
- 4) the cells are non-tumorigenic.
A continuous human liver epithelial cell line isolated from normal adult human liver tissue consisting of cells having the following characteristics:
- 1) the cells have been immortalized by transformation with the transforming genes of a transforming virus selected from the group consisting of SV40 virus, adenovirus, and papilloma compounds to DNA-adduct forming compounds;
- 2) the cells metabolically activate carcinogen precursor compounds to DNA-adduct forming compounds;
- 3) the cells demonstrate a pattern of gene expression, wherein said pattern of gene expression is such that the cells:
- a) do not express .alpha.-fetoprotein;
- and
- b) express mRNA encoding at least one of the proteins selected from the group consisting of a transferrin, .alpha.-1-antitrypsin, .alpha.2 macroglobulin, a catalase, superoxide dismutase and glutathione peroxidase;
- and
- 4) the cells are non-tumorigenic.
The cell line which is the THLE-3 (ATCC CRL 11233) cell line.
Dependencies are inferred from the claim text.