US 5,665,583 A · Methods and materials relating to IMPDH and GMP production

Abstract

Disclosed are purified and isolated DNA sequences encoding eukaryotic proteins possessing biological properties of inosine 5'-monophosphate dehydrogenase ("IMPDH"). Illustratively, mammalian (e.g., human) IMPDH-encoding DNA sequences are useful in transformation or transfection of host cells for the large scale recombinant production of the enzymatically active expression products and/or products (e.g., GMP) resulting from IMPDH catalyzed synthesis in cells. Vectors including IMPDH-encoding DNA sequences are useful in gene amplification procedures. Recombinant proteins and synthetic peptides provided by the invention are useful as immunological reagents and in the preparation of antibodies (including polyclonal and monoclonal antibodies) for quantitative detection of IMPDH.

Claims18

Dependent claims

A purified and isolated nucleic acid segment or its complement comprising at least 20 nucleotides in common with the nucleotide sequence of FIG. 1A, FIG. 1B, FIG. 1C and FIG. 1D;

FIG. 2A, FIG. 2B, FIG. 2C, and FIG. 2D;
or FIG. 3A, FIG. 3B, and FIG. 3C when aligned sequentially, the segment capable of hybridizing to the sequence shown in FIG. 1A, FIG. 1B, FIG. 1C, and FIG. 1D;
FIG. 2A, FIG. 2B, FIG. 2C and FIG. 2D;
or FIG. 3A, FIG. 3B, and FIG. 3C under stringent conditions.

1 more element

Dependencies are inferred from the claim text.

Methods and materials relating to IMPDH and GMP production

Life of this patent

Methods and materials relating to IMPDH and GMP production

Life of this patent

Abstract

Claims18