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US 5,665,577 AGranted
Vectors containing HIV packaging sequences, packaging defective HIV vectors, and uses thereof
AssigneeDana-Farber Cancer Institute, Inc.
InventorsJoseph G. Sodroski, William A. Haseltine, Mark Poznansky, Andrew Lever, Heinrich Gottlinger
Life of this patent
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Abstract
Packaging defective and packaging proficient HIV vectors are disclosed. These vectors can be used to establish HIV packaging defective cell lines, and to package desired genes. These cell lines can be used in developing a vaccine, HIV antibodies and as part of a system for gene transfer. The packaging proficient vector can be used to target HIV target cells.
Claims72
An HIV vector comprising:
- (a) a DNA segment from an HIV genome, wherein the DNA segment comprises the HIV gag, pol and env genes;
- wherein said HIV vector lacks the HIV packaging sequence necessary to package HIV RNA into virions;
- wherein said HIV packaging sequence is the nucleotide sequence located between the 5' major splice donor site and the initiation codon of the gag gene on the HIV genome;
- and
- (b) a promoter operably linked to the DNA segment from an HIV genome of (a);
- wherein the HIV vector, when introduced into a eukaryotic host cell, express HIV gag, pol and env proteins to form HIV virions that do not contain sufficient HIV RNA to result in a replication competent HIV virion.
3 more elements
At least two HIV vectors, wherein each of the at least two HIV vectors has
- (a) a DNA segment encoding a gag or an env gene from an HIV genome but not both, wherein one of the at least two HIV vector or an additional HIV vector has a DNA segment encoding a pol gene from an HIV genome, wherein each of the HIV vectors lacks the HIV packaging sequence necessary to package HIV RNA into virions;
- wherein said HIV packaging sequence is the nucleotide sequence located between the 5' major splice donor site and the initiation codon of the gag gene on the HIV genome;
- (b) a promoter operably linked to each of the DNA segments from an HIV genome of (a);
- and
- (c) a polyadenylation sequence located downstream of each of the DNA segments from an HIV genome of (a);
- wherein the polyadenylation sequence does not comprise a functional LTR sequence;
- wherein the vectors, when introduced into a eukaryotic host cell, express in combination the gag, pol and env proteins to form HIV virions that do not contain sufficient HIV RNA to result in a replication competent HIV virion.
4 more elements
Two HIV vectors, wherein each vector has inserted therein:
- (a) exactly one of two of any of three DNA segments from an HIV genome, wherein the three DNA segments are selected from the group consisting of the HIV gag, pol and env genes;
- wherein each of the two vectors lacks the HIV packaging sequence necessary to package HIV RNA into virions;
- wherein said HIV packaging sequence is the nucleotide sequence located between the 5' major splice donor site and the initiation codon of the gag gene on the HIV genome;
- (b) a promoter operably linked to each of the DNA segments from an HIV genome of (a);
- and
- (c) a polyadenylation sequence located downstream of each of the DNA segments from an HIV genome of (a);
- wherein the polyadenylation sequence does not comprise a functional LTR sequence;
- wherein the two vectors, when introduced into a eukaryotic host cell, express in combination the gag, pol and env proteins to form HIV virions that do not contain sufficient HIV RNA to result in a replication competent HIV virion.
5 more elements
Two HIV vectors wherein
- (a) one HIV vector, referred to as said first vector, contains a DNA segment from an HIV genome consisting of the HIV gag gene or the HIV gag-pol genes, wherein said first vector does not contain a DNA segment containing the HIV env gene, wherein said first vector can optionally contain a DNA segment from an HIV genome consisting of the HIV pol gene,
- (b) the other HIV vector, referred to as said second vector, contains a DNA segment from an HIV genome consisting of the HIV env gene, wherein said second vector does not contain a DNA segment containing the HIV gag gene, wherein said second vector can optionally contain a DNA segment from an HIV genome consisting of the HIV pol gene,
- (c) wherein each of the HIV vectors lacks the HIV packaging sequence necessary to package HIV RNA into virions;
- wherein said HIV packaging sequence is the nucleotide sequence located between the 5' major splice donor site and the initiation codon of the gag gene on the HIV genome;
- (d) a promoter operably linked to each of the DNA segments from an HIV genome of (a) and (b);
- and
- (e) a polyadenylation sequence located downstream of each of the DNA segments from an HIV genome of (a) and (b);
- wherein the polyadenylation sequence does not comprise a functional LTR sequence;
- wherein the two HIV vectors, when introduced into a eukaryotic host cell, express in combination the gag, pol and env proteins to form HIV virions that do not contain sufficient HIV RNA to result in a replication competent HIV virion.
6 more elements
Dependencies are inferred from the claim text.