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US 5,665,576 AGranted
Callus cell induction and the preparation of taxanes
AssigneeE. R. Squibb & Sons, Inc.
InventorsPaul M. Cino, Steven R. Schwarz, Dana L. Cazzulino
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Abstract
A method for the production of taxanes such as taxol. The method includes inducing formation of callus cells by contacting an explant tissue with a liquid medium without complete submergence of the tissue in the medium. The callus cells formed are employed in a liquid suspension cell culture to produce one or more taxanes.
Claims7
A method for the production of at least one taxane, comprising the steps of:
- (A) inducing the formation of callus cells capable of producing at least one taxane, wherein said callus cells are formed from explant tissue according to a method comprising the steps of:
- (i) contacting said explant tissue with a liquid medium without completely submerging said tissue in said medium;
- and
- (ii) holding said explant tissue and liquid medium at conditions suitable for the induction of callus cells, whereby callus cells are formed which are capable of producing at least one taxane;
- (B) culturing said cells under liquid suspension cell culture conditions to produce said taxane(s);
- and
- (C) recovering said at least one taxane.
4 more elements
Dependencies are inferred from the claim text.