Microbial esterase for the enantioselective cleavage of 1-phenylethyl acetate
AssigneeBoehringer Mannheim GmbH
InventorsHans-Willi Krell, Peter Rasor
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Abstract
The invention concerns a microbial esterase which enantioselectively cleaves (S)-1-phenylethyl acetate and is obtainable from Arthrobacter spec. (DSM 7034), Pseudomonas fluorescens (DSM 7033) or Bacillus subtilis (DSM 7035) as well as a process for enantioselectively cleaving a 1-arylalkyl ester of a carboxylic acid and a process for the production of an enantiomerically-pure 1-arylalkanol using this esterase.
Claims6
A purified microbial esterase which enantioselectively cleaves (S)-1-phenylethyl acetate, having molecular weight of about 100,000 daltons when determined by means of native polyacrylamide gel electrophoresis and having an isoelectric point of about 4.7, and which is obtained from Arthrobacter spec.
A process for the isolation of a microbial esterase which enantioselectively cleaves (S)-1-phenylethyl acetate, comprising:
- a) treating a cell lysate from Arthrobacter spec. (DSM 7034) with a soluble ion exchanger;
- and
- b) isolating and purifying the esterase from the solution of step a) by hydrophobic chromatography and ion-exchange chromatography.
Dependencies are inferred from the claim text.