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US 5,665,574 AGranted
Method for preparing N-acetylneuraminic acid
AssigneeMarukin Shoyu Co., Ltd.
InventorsYoji Tsukada, Yasuhiro Ohta
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Abstract
A method for preparing N-acetylneuraminic acid characterized in reacting N-acetylglucosamine with pyruvic acid in an alkaline condition by the action of N-acetylneuraminic acid lyase.
Claims13
A method for preparing N-acetylneuraminic acid comprising:
- reacting N-acetylglucosamine with pyruvic acid at a pH within the range from about 10 to about 12 in the presence of N-acetylneuraminic acid lyase;
- and
- recovering N-acetylneuraminic acid.
The method as claimed in claim 4, wherein the pH is about 10.5.
A method for preparing N-acetylneuraminic acid comprising:
- reacting N-acetylglucosamine with pyruvic acid at a pH within the range from about 8 to about 12 in the presence of N-acetylneuraminic acid lyase, but in the absence of epimerase;
- and
- recovering N-acetylneuraminic acid.
Dependencies are inferred from the claim text.