Method of purifying a population of cells enriched for hematopoietic stem cells populations of cells obtained thereby and methods of use thereof
AssigneesSystemix, Inc., The Toronto Hospital Research Institute
InventorsLesley Murray, D. Robert Sutherland
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Abstract
The invention relates to methods of obtaining compositions enriched in hematopoietic stem cells by separating out an enriched fraction of cells expressing the marker CDw109. Methods of obtaining compositions enriched in hematopoietic megakaryocyte progenitor cells are also provided. Compositions enriched for stem cells and populations of cells obtained therefrom are also provided by the invention. Methods of use of the cells are also included.
Claims21
A method of obtaining a composition substantially enriched in a subpopulation of hematopoietic stem cells comprising:
- obtaining a source of human hematopoietic cells;
- separating a population of human hematopoietic cells from the source;
- contacting the population of human hematopoietic cells with a monoclonal antibody specific for the marker CDw109;
- and
- separating the subpopulation that is specifically bound by the monoclonal antibody thereby obtaining a composition substantially enriched in a subpopulation of human hematopoietic stem cells.
A method of obtaining a composition substantially enriched in a subpopulation of hematopoietic megakaryocyte progenitor cells comprising:
- obtaining a source of hematopoietic cells;
- separating a population of human hematopoietic cells from the source;
- contacting a population of human hematopoietic cells with a monoclonal antibody specific for the marker CDw109:
- and
- separating the subpopulation that is specifically bound by the monoclonal antibody, thereby obtaining a composition substantially enriched in the subpopulation of human hematopoietic megakaryocyte progenitor cells.
A method for expanding hematopoietic megakaryocyte progenitor cells comprising:
- obtaining a source of human hematopoietic cells:
- separating a population of human hematopoietic cells from the source;
- contacting the population of human hematopoietic cells with a monoclonal antibody specific for the marker CDw 109:
- and
- separating the subpopulation that is specifically bound by the monoclonal antibody;
- and
- culturing the subpopulation in the presence of an effective amount of at least one cytokine effective in inducing expansion of megakaryocyte progenitor cells, thereby expanding hematopoietic megakaryocyte progenitor cells.
A composition comprising hematopoietic cells enriched for megakaryocyte progenitor cells wherein the cells express CDw109 and CD34.
Dependencies are inferred from the claim text.