Magnetic bead precipitation method
AssigneeAmersham International plc
InventorsMichael Alan Reeve, Philip Steven Robinson
Life of this patent
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Abstract
A method of recovering a first material, e.g. plasmid DNA, from a solution containing pre-precipitated second material, e.g. genomic DNA and cellular debris, by precipitating the first material in the presence of magnetically attractable beads which become non-specifically associated with the newly formed precipitate, using a magnet to draw down the beads and the newly formed precipitate, and removing the supernatant.
Claims7
A method of recovering a first molecular species from a starting liquid containing in solution the first molecular species in admixture with a second molecular species, which method comprises the steps of:
- precipitating the second molecular species to form an aggregated precipitate of the second molecular species in the liquid,
- adding magnetically attractable beads to form a suspension of the beads in the liquid,
- precipitating the first molecular species to form an aggregated precipitate of the first molecular species which entraps the magnetically attractable beads,
- applying a magnetic field to draw down the magnetically attractable beads and the associated aggregated precipitate of the first molecular species,
- removing the supernatant liquid containing the aggregated precipitate of the second molecular species.
Dependencies are inferred from the claim text.