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US 5,665,544 AGranted
RNA fingerprinting to determine RNA population differences
AssigneeAmersham International plc
InventorsAlexander Anatoljevich Chenchik, Ludmila Borisovna Diachenko, Robert Shavlovich Beabealashvili, Christopher John Carter
Life of this patent
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Abstract
A method of producing an RNA fingerprint of a poly(A).sup.+ RNA preparation. At least one oligonucleotide primer and a supply of nucleotides of which one is a terminator nucleotide, are added to the poly(A).sup.+ RNA preparation or a single-stranded cDNA preparation derived from it. cDNA products are synthesized from the RNA, or DNA products from the cDNA and the products are separated to produce an RNA fingerprint.
Claims12
A method of producing a fingerprint of a eukaryotic poly(A)+ RNA preparation comprising;
- adding to the poly(A)+ RNA preparation or to a single-stranded cDNA preparation derived therefrom at least one oligonucleotide primer and four different nucleotides, wherein three of the nucleotides permit elongation and one of the nucleotides is a chain terminator;
- synthesizing cDNA products from said RNA preparation or DNA products from said single-stranded cDNA preparation;
- and
- separating said cDNA products or DNA products to produce a fingerprint.
1 more element
Dependencies are inferred from the claim text.