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US 5,665,542 AGranted
Toxoplasma gondii P28 gene and methods for its use
AssigneeResearch Institute of Palo Alto Medical Foundation
InventorsJeffrey B. Prince, Fausto G. De Araujo, Somesh D. Sharma, Jack S. Remington
Life of this patent
Estimating the term…
Abstract
Genetic material encoding the P28 peptide of Toxoplasma gondii has been isolated and characterized. This genetic material allows the production of peptides for use in diagnosis or immunization or can itself be directly used in hybridization assays.
Claims22
An isolated DNA or RNA molecule, which contains a nucleotide coding sequence encoding Toxoplasma gondii protein P28 or a complementary DNA or RNA sequence that specifically hybridizes with said Toxoplasma gondii protein P28 sequence.
An isolated polynucleotide, containing at least 10 consecutive nucleotides selected from the group of nucleotide sequences consisting of:
- (A)
- __________________________________________________________________________ CTGCAGACCA GATGTCGCAT TTGAAGGTTA CATCTCGCTT GAAGACTTCC GGTGCCATGT ATAAGGCAGT GCCAGCCGCA TTAGTGCTGT GTTCGTCGCT CTTGAAAAGT TCTGCGAGAC CGAAGTCGAT AATTTTAATC GGAGAGTGAG GTGAAGTGTC TTGAAAGAGA ATGTTTTCCG GCTTGAGGTC TCTGAGGCAG AAACGCGACA TGCAGATGGA ACGGCGAACG CCTTTGAACC CAAACGAATC GCGAAGCTCC AGTGAACTGG ACAAGCAAAT TTGCAATTCG CGTCGTTATC GCACGTTGTT TCTCTTCCCA CGAATAGTTG TTTTGATTAG ATATTGCTTC TTCTCCACAT ATCGCCTCAC AATGTTCGCC GTAAAACATT GTTTGCTGGT TGTTGCCGTT GGCGCCCTGG TCAACGTCTC GGTGAGGGCT GCCGAGTTTT CCGGAGTTGT TAACCAGGGA CCAGTCGACG TGCCTTTCAG CGGTAAACCT CTTGATGAGA GAGCAGTTGG GTAAGTTGGC AAAAGTAATG ATAGAGGCAG GGGTTGAACG ATAGGCGGCT GCAGATTTGT ATAACACAAC ATGATGTAGC TGCCACGGTT TTTTTTCGGA GAGTGATGCC GTCTGACTGT TCATCGCACC CATGGGAGCT AGGGAGGTGC GCTTTCTGTG TGATATGTAT TGTCCTAGTC CAATTTCCCA CGCACTGTAG TGTCTTGAGA CTCGGTGCCA TGTAGAATTT TGTGTCTGCC TGCAGAGGAA AAGGTGAACA TACACCACCA CTCCCAGACG AGAGGCAACA AGAGCCAGAA GAACCGGTTT CCCAACGTGC ATCCAGAGTG GCAGAACAAC TGTTTCGCAA GTTCTTGAAG TTCGCTGAAA ACGTCGGACA TCACAGTGAG AAGGCCTTCA AAAAAGCAAA GGTGGTGGCA GAAAAAGGCT TCACCGCGGC AAAAACGCAC ACGGTTAGGG GTTTCAAGGT GGCCAAAGAA GCAGCTGGAA GGGGCATGGT GACCGTTGGC AAGAAACTCG CGAATGTGGA GAGTGACAGA AGCACTACGA CAACGCAGGC CCCCGACAGC CCTAATGGCC TGGCAGAAAC CGAGGTTCCA GTGGAGCCCC AACAGCGGGC CGCACACGTG CCCGTCCCAG ACTTTTCGCA GTAATGTTGA CTACGACGAA AGTGATGCGC AGGCTGGAAA GCCGCTGAAG GGAGAAGTCT ACAAAGCCGA TCAGTGAAAA ATGTGTGGGG AGGTGGTCTT GTTGCAGGAA TGCAATGGTG TTAAGC, __________________________________________________________________________
- (B) a DNA sequence encoding the same amino acids as the sequence of (A);
- (C) an RNA sequence corresponding to the sequence of (A), wherein every T is replaced with U;
- (D) an RNA sequence corresponding to the sequence of (B), wherein every T is replaced with U;
- (E) a DNA sequence complementary to the sequence of (A) that specifically hybridizes to the sequence of (A);
- (F) a DNA sequence complementary to the sequence of (B) that specifically hybridizes to the sequence of (B);
- (G) an RNA sequence corresponding to the sequence of (E), wherein every T is replaced with U;
- and
- (H) an RNA sequence corresponding to the sequence of (F), wherein every T is replaced with U.
7 more elements
An isolated nucleic acid molecule, wherein said molecule contains a coding sequence selected from the group consisting of:
- (A)
- __________________________________________________________________________ ATGTTTTCCG GCTTGAGGTC TCTGAGGCAG AAACGCGACA TGCAGATGGA ACGGCGAACG CCTTTGAACC CAAACGAATC GCGAAGCTCC AGTGAACTGG ACAAGCAAAT TTGCAATTCG CGTCGTTATC GCACGTTGTT TCTCTTCCCA CGAATAGTTG TTTTGATTAG ATATTGCTTC TTCTCCACAT ATCGCCTCAC AATGTTCGCC GTAAAACATT GTTTGCTGGT TGTTGCCGTT GGCGCCCTGG TCAACGTCTC GGTGAGGGCT GCCGAGTTTT CCGGAGTTGT TAACCAGGGA CCAGTCGACG TGCCTTTCAG CGGTAAACCT CTTGATGAGA GAGCAGTTGG A GGAAAAGGTG AACATACACC ACCACTCCCA GACGAGAGGC AACAAGAGCC AGAAGAACCG GTTTCCCAAC GTGCATCCAG AGTGGCAGAA CAACTGTTTC GCAAGTTCTT GAAGTTCGCT GAAAACGTCG GACATCACAG TGAGAAGGCC TTCAAAAAAG CAAAGGTGGT GGCAGAAAAA GGCTTCACCG CGGCAAAAAC GCACACGGTT AGGGGTTTCA AGGTGGCCAA AGAAGCAGCT GGAAGGGGCA TGGTGACCGT TGGCAAGAAA CTCGCGAATG TGGAGAGTGA CAGAAGCACT ACGACAACGC AGGCCCCCGA CAGCCCTAAT GGCCTGGCAG AAACCGAGGT TCCAGTGGAG CCCCAACAGC GGGCCGCACA CGTGCCCGTC CCAGACTTTT CGCAG __________________________________________________________________________
- (B) an RNA sequence corresponding to the sequence of (A) wherein every I is replaced with U;
- (C) a DNA sequence complementary to the sequence of (A) that specifically hybridizes to the sequence of (A);
- and
- (D) an RNA sequence corresponding to the sequence of (C), wherein every T is replaced with U.
3 more elements
An isolated oligonucleotide, containing at least 10 consecutive nucleotides selected from a the group of nucleotide sequences consisting of:
- (A)
- __________________________________________________________________________ ATGTTTTCCG GCTTGAGGTC TCTGAGGCAG AAACGCGACA TGCAGATGGA ACGGCGAACG CCTTTGAACC CAAACGAATC GCGAAGCTCC AGTGAACTGG ACAAGCAAAT TTGCAATTCG CGTCGTTATC GCACGTTGTT TCTCTTCCCA CGAATAGTTG TTTTGATTAG ATATTGCTTC TTCTCCACAT ATCGCCTCAC AATGTTCGCC GTAAAACATT GTTTGCTGGT TGTTGCCGTT GGCGCCCTGG TCAACGTCTC GGTGAGGGCT GCCGAGTTTT CCGGAGTTGT TAACCAGGGA CCAGTCGACG TGCCTTTCAG CGGTAAACCT CTTGATGAGA GAGCAGTTGGA GGAAAAGGTG AACATACACC ACCACTCCCA GACGAGAGGC AACAAGAGCC AGAAGAACCG GTTTCCCAAC GTGCATCCAG AGTGGCAGAA CAACTGTTTC GCAAGTTCTT GAAGTTCGCT GAAAACGTCG GACATCACAG TGAGAAGGCC TTCAAAAAAG CAAAGGTGGT GGCAGAAAAA GGCTTCACCG CGGCAAAAAC GCACACGGTT AGGGGTTTCA AGGTGGCCAA AGAAGCAGCT GGAAGGGGCA TGGTGACCGT TGGCAAGAAA CTCGCGAATG TGGAGAGTGA CAGAAGCACT ACGACAACGC AGGCCCCCGA CAGCCCTAAT GGCCTGGCAG AAACCGAGGT TCCAGTGGAG CCCCAACAGC GGGCCGCACA CGTGCCCGTC CCAGACTTTT CGCAG __________________________________________________________________________
- (B) a DNA sequence encoding the same amino acids as the sequence of (A);
- (C) an RNA sequence corresponding to the sequence of (A), wherein every T is replaced with U;
- (D) an RNA sequence corresponding to the sequence of (B), wherein every T is replaced with U;
- (E) a DNA sequence complementary to the sequence of (A) that specifically hybridizes to the sequence of (A);
- (F) a DNA sequence complementary to the sequence of (B) that specifically hybridizes to the sequence of (B);
- (G) an RNA sequence corresponding to the sequence of (E), wherein every T is replaced with U;
- and
- (H) an RNA sequence corresponding to the sequence of (F), wherein every T is replaced with U.
7 more elements
An isolated polynucleotide, comprising at least 10 consecutive nucleotides selected from the group of nucleotide of sequences consisting of:
- (A)
- __________________________________________________________________________ TTCGCC GTAAAACATT GTTTGCTGGT TGTTGCCGTT GGCGCCCTGG TCAACGTCTC GGTGAGGGCT GCCGAGTTTT CCGGAGTTGT TAACCAGGGA CCAGTCGACG TGCCTTTCAG CGGTAAACCT CTTGATGAGA GAGCAGTTGGA GGAAAAGGTG AACATACACC ACCACTCCCA GACGAGAGGC AACAAGAGCC AGAAGAACCG GTTTCCCAAC GTGCATCCAG AGTGGCAGAA CAACTGTTTC GCAAGTTCTT GAAGTTCGCT GAAAACGTCG GACATCACAG TGAGAAGGCC TTCAAAAAAG CAAAGGTGGT GGCAGAAAAA GGCTTCACCG CGGCAAAAAC GCACACGGTT AGGGGTTTCA AGGTGGCCAA AGAAGCAGCT GGAAGGGGCA TGGTGACCGT TGGCAAGAAA CTCGCGAATG TGGAGAGTGA CAGAAGCACT ACGACAACGC AGGCCCCCGA CAGCCCTAAT GGCCTGGCAG AAACCGAGGT CCAGTGGAGC CCCAACAGCG GGCCGCACAC GTGCCCGTCC CAGACTTTTC GCAG, __________________________________________________________________________
- (B) a DNA sequence encoding the same amino acids as the sequence of (A);
- (C) an RNA sequence corresponding to the sequence of (A), wherein every T is replaced with U;
- (D) an RNA sequence corresponding to the sequence of (B), wherein every T is replaced with U;
- (E) a DNA sequence complementary to the sequence of (A) that specifically hybridizes to the sequence of (A);
- (F) a DNA sequence complementary to the sequence of (B) that specifically hybridizes to the sequence of (B);
- (G) an RNA sequence corresponding to the sequence of (E), wherein every T is replaced with U;
- and
- (H) an RNA sequence corresponding to the sequence of F), wherein every T is replaced with U.
7 more elements
An isolated polynucleotide, comprising at least 10 consecutive nucleotides selected from the group of nucleotide sequences consisting of:
- (A)
- __________________________________________________________________________ TTCGCC GTAAAACATT GTTTGCTGGT TGTTGCCGTT GGCGCCCTGG TCAACGTCTC GGTGAGGGCT GCCGAGTTTT CCGGAGTTGT TAACCAGGGA CCAGTCGACG TGCCTTTCAG CGGTAAACCT CTTGATGAGA GAGCAGTTGG GTAAGTTGGC AAAAGTAATG ATAGAGGCAG GGGTTGAACG ATAGGCGGCT GCAGATTTGT ATAACACAAC ATGATGTAGC TGCCACGGTT TTTTTTCGGA GAGTGATGCC GTCTGACTGT TCATCGCACC CATGGGAGCT AGGGAGGTGC GCTTTCTGTG TGATATGTAT TGTCCTAGTC CAATTTCCCA CGCACTGTAG TGTCTTGAGA CTCGGTGCCA TGTAGAATTT TGTGTCTGCC TGCAGAGGAA AAGGTGAACA TACACCACCA CTCCCAGACG AGAGGCAACA AGAGCCAGAA GAACCGGTTT CCCAACGTGC ATCCAGAGTG GCAGAACAAC TGTTTCGCAA GTTCTTGAAG TTCGCTGAAA ACGTCGGACA TCACAGTGAG AAGGCCTTCA AAAAAGCAAA GGTGGTGGCA GAAAAAGGCT TCACCGCGGC AAAAACGCAC ACGGTTAGGG GTTTCAAGGT GGCCAAAGAA GCAGCTGGAA GGGGCATGGT GACCGTTGGC AAGAAACTCG CGAATGTGGA GAGTGACAGA AGCACTACGA CAACGCAGGC CCCCGACAGC CCTAATGGCC TGGCAGAAAC CGAGGTTCCA GTGGAGCCCC AACAGCGGGC CGCACACGTG CCCGTCCCAG ACTTTTCGCA G __________________________________________________________________________
- (B) a DNA sequence encoding the same amino acids as the sequence of (A);
- (C) an RNA sequence corresponding to the sequence of (A), wherein every T is replaced with U;
- (D) an RNA sequence corresponding to the sequence of (B), wherein every T is replaced with U;
- (E) a DNA sequence complementary to the sequence of (A) that specifically hybridizes to the sequence of (A);
- (F) a DNA sequence complementary to the sequence of (B) that specifically hybridizes to the sequence of (B);
- (G) an RNA sequence corresponding to the sequence of (E), wherein every T is replaced with U;
- and
- (H) an RNA sequence corresponding to the sequence of (F), wherein every T is replaced with U.
7 more elements
Dependencies are inferred from the claim text.