Ultrasensitive microtrace procedure for monitoring the origin of a material
OwnerJames Howard Slater, John Edward Mintoninventors
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Abstract
A method of monitoring the movement of a material which comprises adding to the material, as a microtrace additive, DNA molecules, sampling the resulting material after movement thereof and detecting the presence of said microtrace additive in the sample. The method is particularly suitable for use in monitoring the movement of oil shipments and the microtrace additive is selected such that it will remain in the oil phase in the event the oil is dispersed in water e.g. sea water.
Claims10
A method of monitoring the movement of a petroleum material in an aqueous medium comprising,
- adding a microtrace additive to the petroleum material, wherein the microtrace additive is DNA in a final concentration in the range of 0.01 to 1000 pg/DNA/.mu.l petroleum material, and wherein the DNA is formulated to be soluble in said petroleum material such that the overall hydrophobicity of the microtrace additive causes it to partition into the petroleum material, and wherein said formulation ensures that the DNA is dissolved in or dispersed within the petroleum material such that it essentially cannot be removed by aqueous washing;
- sampling the microtrace additive-containing petroleum material after movement thereof;
- removing the microtrace additive from the petroleum material;
- and
- detecting the DNA microtrace additive by means of an amplification reaction.
A composition consisting essentially of a microtrace additive dissolved in or dispersed in a petroleum material, wherein said microtrace additive is DNA in a final concentration in the range of 0.01 to 1000 pg/DNA/.mu.l petroleum material, and wherein the DNA is formulated to be soluble in said petroleum material such that the overall hydrophobicity of the microtrace additive causes it to partition into the petroleum material, and wherein said formulation ensures that the DNA is dissolved in or dispersed within the petroleum material such that it essentially cannot be removed by aqueous washing.
Dependencies are inferred from the claim text.