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US 5,665,366 AGranted
Skin care method and composition
AssigneeElizabeth Arden Co., Division of Conopco, Inc.
InventorsAnthony Vincent Rawlings, Allan Watkinson
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AssigneeElizabeth Arden Co., Division of Conopco, Inc.
InventorsAnthony Vincent Rawlings, Allan Watkinson
Estimating the term…
A composition for topical application to the skin for alleviation or prevention of dry flaky skin conditions, dandruff or ache comprising one or more stratum corneum trypsin-like enzymes. The composition may further comprise an additional enzyme selected from glycosidases, other proteases, lipases and mixtures thereof. Optional additional active ingredients include sunscreens, lipids, hydroxy carboxylic acids and ketocarboxylic acids.
A method of relieving or ameliorating dry skin, acne and for smoothening skin which by topical application to the skin of a composition consisting essentially of a stratum corneum serine protease which in its active form is inhibited by antipain and leupeptin and is capable of decomposing the substrate boc-phe-ser-arg-aminomethylcoumarin.
A method of relieving or ameliorating dry skin, acne and for smoothening skin by topical application to the skin of a composition consisting essentially of:
A method of relieving or ameliorating dry skin, acne and for smoothening skin by topical application to the skin of a composition consisting essentially of:
Dependencies are inferred from the claim text.
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Proteins; Peptides; Derivatives or degradation products thereof: enzymes
Hydrolases: acting on ester bonds , e.g. lipases, ribonucleases
Hydrolases: acting on glycosyl compounds , e.g. cellulases, lactases
Hydrolases: acting on carbon-nitrogen bonds, other than peptide bonds , e.g. asparaginase
Term ended 12 Sep 2014 (estimated)
USPTO file history isn’t available for this patent, so only indexed dates are shown.
The present invention relates to compositions for topical application to the skin and their cosmetic and pharmaceutical use. In particular, the invention relates to compositions comprising stratum corneum trypsin-like enzymes and their use in alleviating or preventing conditions involving abnormal desquamation by facilitating desmosomal degradation.
In normal, healthy epidermis the continuous production of new stratum corneum is balanced by a well-regulated shedding of corneocytes from the skin surface. Little is known about this desquamation process at the molecular level.
It has been shown by A. Lundstrom and T. Egelrud (J. Invest Dermatol, (1988) 91 340-343; Arch Dermatol Res (1990) 282 234-237; J. Invest Dermatol (1990) 94 216-220) that cohesion between cells in the stratum corneum is dependant on protein structures. These structures must be degraded before cell dissociation can occur.
FIG. 1 depicts fractionation profile of stratum corneum extract.
FIG. 2 depicts a cation exchange chromatography profile of 29 kDa fraction (having boc-phe-ser-arg-AMC activity).
FIG. 3 depicts an inhibition profile of stratum corneum 29 kDa trypsin-like enzyme.
As used herein, the term "stratum corneum trypsin-like enzyme" means a stratum corneum serine protease, or a pro-enzyme thereof, which in its active form exhibits similar substrate sensitivity and inhibitor sensitivity to trypsin. More specifically, the term "stratum corneum trypsin-like enzyme" means a serine protease, or a pro-enzyme thereof, which in its active form is inhibited by antipain, and leupeptin. More particularly, the term "stratum corneum trypsin-like enzyme" means an enzyme which in its active form is capable of decomposing the substrate boc-phe-ser-arg-aminomethylcoumarin (AMC).
It will be appreciated that a pro-enzyme is an inactive form of the enzyme which may be activated by appropriate proteolytic cleavage to give the active form.
Suitable stratum corneum trypsin-like enzymes for use according to the present invention have apparent molecular weights, as determined by the method of comparing the electrophoretic mobility with standard proteins on sodium dodecyl sulphate polyacrylamide gel electrophoresis of 24 kDa, 26 kDa and 27 kDa.