Recombinant baculovirus with insecticidal activity
AssigneeNorth Carolina State University
InventorsCharles S. Levings, III, Kenneth L. Korth
Life of this patent
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Abstract
A method of agricultural pest control using recombinant baculovirus which express DNA coding for the mitochondrial pore-forming protein URF13, or homologous DNA. Compositions for agricultural use containing the recombinant baculovirus.
Claims34
A method of reducing the population of an insect in an agricultural field comprising applying to the field a recombinant baculovirus capable of producing polyhedrin protein and which is able to infect the insect, said recombinant baculovirus applied in an amount effective to reduce the population of the insect, and said baculovirus containing and expressing heterologous DNA selected from the group consisting of:
- (a) isolated DNA which encodes the maize mitochondrial pore-forming protein URF13;
- (b) isolated DNA which hybridizes to isolated DNA of (a) above under conditions represented by a wash stringency of 0.3M NaCl, 0.03M sodium citrate, and 0.1% SDS at 60.degree. C., and which encodes a membrane protein;
- and
- (c) isolated DNA differing from the isolated DNAs of (a) and (b) above in codon sequence due to the degeneracy of the genetic code, and which encodes the protein encoded by DNA of (a) or (b) above.
A composition for reducing the population of an insect in an agricultural field, comprising an effective insect-population reducing amount of a recombinant baculovirus capable of producing a polyhedrin protein, said baculovirus capable of infecting the insect and containing and expressing heterologous DNA selected from the group consisting of:
- (a) isolated DNA which encodes the maize mitochondrial pore-forming protein URF13;
- (b) isolated DNA which hybridizes to isolated DNA of (a) above under conditions represented by a wash stringency of 0.3M NaCl, 0.03M sodium citrate, and 0.1% SDS at 60.degree. C., and which encodes a membrane protein;
- and
- (c) isolated DNA differing from the isolated DNAs of (a) and (b) above in codon sequence due to the degeneracy of the genetic code, and which encodes the protein encoded by DNA of (a) or (b) above.
A method of reducing the population of an insect in an agricultural field comprising applying to the field a recombinant nuclear polyhedrosis baculovirus which is able to infect the insect, said recombinant nuclear polyhedrosis baculovirus applied in an amount effective to reduce the population of the insect, and said recombinant nuclear polyhedrosis baculovirus containing and expressing heterologous DNA selected from the group consisting of:
- (a) isolated DNA which encodes the maize mitochondrial pore-forming protein URF13;
- (b) isolated DNA which hybridizes to isolated DNA of (a) above under conditions represented by a wash stringency of 0.3M NaCl, 0.03M sodium citrate, and 0.1% SDS at 60.degree. C., and which encodes a membrane protein;
- and
- (c) isolated DNA differing from the isolated DNAs of (a) and (b) above in codon sequence due to the degeneracy of the genetic code, and which encodes the protein encoded by DNA of (a) or (b) above.
Dependencies are inferred from the claim text.