Soybean peroxidase electrochemical sensor
AssigneeE. Heller & Company
InventorsAdam Heller, Mark S. Vreeke
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Abstract
An electrochemical biosensor able to measure hydrogen peroxide which is stable at temperatures near 37.degree. C. or in excess of 37.degree. C. for sustained periods of time and in biological environments. The electrochemical biosensor contains a thermostable peroxidase, preferably derived from soybean, and is useful in analytical biosensors.
Claims19
An electrochemical hydrogen peroxide sensor comprising:
- a non-corroding electrode;
- and
- thermostable soybean peroxidase immobilized on the non-corroding electrode and electrically connected to the non-corroding electrode,
- the sensor having no leachable components, and being capable of operation at temperatures of 37.degree. C. or higher with less than 2% loss of sensitivity per hour of continuous operation.
A method for the electrochemical analysis of hydrogen peroxide, said method comprising the steps of:
- generating an electrical signal by an electrode in the presence of hydrogen peroxide, the electrode comprising a thermostable soybean peroxidase coimmobilized with a non-leachable redox compound on a non-corroding electrode;
- and,
- correlating the generated electrical signal with the presence of hydrogen peroxide in the sample.
A method for continuous measurement of analyte, comprising the steps of:
- placing a sensor on or in a body fluid or tissue, the sensor comprising a thermostable soybean peroxidase electrically connected to a non-corroding electrode and co-immobilized with a non-leachable redox compound onto the electrode and a second enzyme immobilized on the electrode,
- the second enzyme catalyzing a reaction which generates hydrogen peroxide in the presence of a specified analyte, and
- correlating an electrical signal generated by the electrode with the amount of analyte present in the body fluid or tissue.
A method for monitoring glucose concentration in vivo, comprising the steps of:
- placing in a subject's body fluid or tissue
- a sensor comprising a thermostable soybean peroxidase electrically connected to a non-corroding electrode and co-immobilized with a non-leachable redox compound onto the electrode, and glucose oxidase immobilized onto the electrode;
- and
- correlating an electrical signal generated by the electrode with the amount of glucose present in the body fluid or tissue.
A method for monitoring the concentration of an analyte in a bioreactor operating at temperatures of 37.degree. C. or greater, comprising the steps of:
- placing in the bioreactor, in contact with the bioreactor's contents, a sensor comprising a thermostable soybean peroxidase coimmobilized with a non-leachable redox compound onto a non-corroding electrode;
- and
- correlating an electrical signal generated by the electrode with the amount of analyte in the bioreactor.
A method for detecting hybridization of a nucleic acid probe to a sample nucleic acid, comprising the steps of:
- immobilizing a sample nucleic acid on a non-corroding electrode;
- incubating the sample nucleic acid-electrode with a nucleic acid probe having bound thereto a thermostable soybean peroxidase, under hybridization conditions;
- and
- correlating an electrical signal produced by the electrode in the presence of added hydrogen peroxide with hybridization of the nucleic acid probe to the sample nucleic acid.
A method for analyzing the composition of a sample nucleic sequence comprising the steps of:
- immobilizing a sample nucleic acid on a non-corroding electrode;
- incubating the sample nucleic acid-electrode with a nucleic acid probe having bound thereto a thermostable soybean peroxidase, under hybridization conditions;
- and
- correlating an electrical signal produced by the electrode in the presence of added hydrogen peroxide with hybridization of the nucleic acid probe to the sample nucleic acid and to the composition of the sample nucleic acid sequence.
A method for analyzing the composition of a sample nucleic sequence comprising the steps of:
- immobilizing a sample nucleic acid on a non-corroding electrode;
- incubating the sample nucleic acid-electrode with a nucleic acid probe having bound thereto a thermostable soybean peroxidase, under varying hybridization conditions;
- and
- correlating changes in electrical signals produced by the electrode in the presence of added hydrogen peroxide under the varying hybridization conditions with variation in hybridization of the nucleic acid probe to the sample nucleic acid and to the composition of the sample nucleic acid sequence.
Dependencies are inferred from the claim text.