Capillary column for high performance electrophoretic separation and detection of SDS proteins and system and using the same
AssigneeNortheastern University
InventorsBarry L. Karger, Katalin Ganzler, Aharon S. Cohen
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Abstract
A capillary column for high performance electrophoretic separation and detection of SDS proteins and system for using the same is disclosed. A preferred column includes a capillary, a static or dynamic layer of coating material on the inner surface of the capillary wall, and a hydrophilic polymer network which is UV-transparent in the tube, filling it. The capillary column is particularly useful in any electrophoresis system requiring UV-transparent materials.
Claims7
A capillary column containing a UV-transparent polymer network for high performance electrophoretic separation and high sensitivity detection of SDS-proteins comprising:
- a capillary having an interior cavity and a wall with an inner surface;
- a layer of coating material on said inner surface of said wall;
- a UV-transparent hydrophilic polymer network filling said interior cavity;
- and
- a UV-transparent buffer, said buffer being selected from a group consisting of Tris-Ches, MES-Na, and AMPO-cacodylic acid (CACO).
A capillary column containing a UV-transparent polymer network for high performance electrophoretic separation and high sensitivity detection of SDS-proteins comprising:
- a capillary having an interior cavity and a wall with an inner surface;
- a layer of coating material on said inner surface of said wall;
- a UV-transparent hydrophilic polymer network filling said interior cavity, said polymer network comprising approximately at least 5% dextran;
- and
- a UV-transparent buffer, said buffer being selected from a group consisting of Tris-Ches, MES-Na, and AMPO-cacodylic acid (CACO).
Dependencies are inferred from the claim text.