Transgenic mice expressing human cholesterol 7.alpha.hydroxylase
AssigneeNortheastern Ohio Universities College of Medicine
InventorJohn Young Ling Chiang
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Abstract
Genomic DNA of cholesterol 7.alpha.-hydroxylase and a minigene are disclosed. The minigene is used for making a transgenic animal that produces functionally active cholesterol 7.alpha.-hydroxylase and functions as a disease model. A cholesterol 7.alpha.-hydroxylase promoter region and reporter gene construct is provided, as well as a transgenic animal that expresses the promoter/reporter gene.
Claims10
A method of making a transgenic mouse that expresses human cholesterol 7.alpha.-hydroxylase (CYP7) in liver tissue in an amount higher than that of normal murine CYP7 expression in an unmodified, non-transgenic mouse comprising the steps of:
- (A) microinjecting a fertilized oocyte of a mouse with a human CYP7 minigene construct comprising a promoter region operably linked to CYP7 nucleotide fragments comprising exon I, intron I, exon II, intron II, exon III, exon IV, exon V, and exon VI, and
- (B) detecting expression of human cholesterol 7.alpha.-hydroxylase (CYP7) in liver tissue of a mouse generated from said fertilized oocyte.
A transgenic mouse which expresses human cholesterol 7.alpha.-hydroxylase (CYP7), wherein said human CYP7 is expressed in liver tissue in an amount higher than that of normal murine CYP7 expression in an unmodified, non-transgenic mouse.
Dependencies are inferred from the claim text.