Modified lambda bacteriophage
AssigneeMedical Science Partners
InventorsBryan L. Ray, Edmund C. C. Lin, Roberto Crea
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Abstract
Disclosed is a protein construct including a chemically modified lambdoid tail protein having a chemically reactive amino acid residue linked to a target molecule. Also disclosed is an infective lambdoid bacteriophage displaying on its outer surface the chemically modified tail protein. In addition, methods of detecting a molecule-of-interest in a solution and methods of detecting cells producing a molecule-of-interest which utilize the infective lambdoid bacteriophage having the chemically modified tail protein are disclosed.
Claims5
An infective bacteriophage lambda comprising a head and a tail, the tail having on its outer surface a modified gpV protein, the modified gpV protein having the sequence set forth in SEQ ID NO:1, wherein a chemically reactive amino acid residue selected from the group consisting of histidine, cysteine, tryptophan, and tyrosine is substituted at amino acid position 246, and wherein the tail has a proteinaceous target molecule chemically coupled to said chemically reactive amine acid residue.
Dependencies are inferred from the claim text.