P0438, a new calcium-regulated promoter
AssigneeLaboratories Serono SA
InventorsAntonioDaza Ortega, Jose Antonio Gil, Tomas Vigal Garcia, Juan Francisco Martin
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Abstract
There is disclosed a new calcium-regulated promoter to be used for increasing production of extracellular enzymes, or heterologous polypeptide, a recombinant vector that includes the DNA sequence of the promoter operatively linked to a DNA encoding said enzyme or polypeptide and a host organism transformed with the recombinant vector that includes the promoter operatively linked to the DNA encoding said enzyme or polypeptide. The present invention further relates to Streptomyces expression systems and methods for expressing foreign DNA sequences in Streptomyces and for secreting to the surrounding medium polypeptides and proteins coded for by those foreign DNA sequences.
Claims3
A method of expressing a foreign DNA sequence in Streptomyces comprising operatively linking said foreign DNA sequence to a Streptomyces expression control sequence which comprises the calcium regulated promoter contained in SEQ ID NO:1 or to a fragment thereof having calcium regulated promoter activity.
Dependencies are inferred from the claim text.