Disposable device in diagnostic assays

A disposable assay device for use in analyte detection in a fluid sample, said device comprising:

• a housing comprising top and bottom plates fastened together, wherein said fastened together top and bottom plates comprise:
• a sample addition port in fluid communication with a main channel comprising in a first direction of fluid flow from said sample addition port:
• a main reagent area comprising agitation means;
• a transport channel;
• an incubation area;
• and
• a waste area;
• wherein said main channel comprises at least one capillary valve upstream from said incubation area;
• a side reagent channel on either side of said main channel, wherein each of said side reagent channels comprised in a second direction of fluid flow:
• a liquid addition port;
• a side reagent area comprising agitation means;
• and
• a side reagent transport channel, wherein said side reagent transport channel is in fluid communication with said main channel at a region upstream from said waste area;
• an optically clear window above said incubation area;
• and
• members of a signal producing system in at least one of said main reagent channel and side reagent channels, wherein said signal producing system produces an optical signal which is related to the presence of analyte in said fluid sample.

A disposable assay device for use in analyte detection in a fluid sample, said device comprising:

• a housing comprising a top and bottom plate fastened together, wherein said top and bottom fastened together plates comprise:
• at least one sample addition port, wherein said sample addition port is in fluid communication with an assay path, wherein said assay path comprises a main channel and one side channel on either side of said main channel, wherein at least a portion of said main channel is a capillary, said main channel comprising in a first direction of fluid flow from said sample addition port:
• a main reagent area comprising an impeller;
• a main transport channel;
• an incubation area;
• a waste area, wherein said waste area comprises a means for enhancing fluid flow through said device;
• and
• at least one capillary valve in said main channel at a region upstream from said incubation area;
• and
• said two side channels comprising in a second direction of fluid flow:
• a liquid addition port;
• a side reagent area comprising an impeller;
• and
• a side reagent transport channel, wherein said side reagent transport channel is in fluid communication with said main transport channel, wherein at least one of said side reagent channels further comprises at least one capillary valve upstream from said main channel;
• an optically clear window above said incubation area, and
• members of a signal producing system in at least one of said main channel and side reagent channel, wherein said signal producing system produces an optical signal which is related to the presence of analyte in said fluid sample.