Immortalized human cell lines containing exogenous cytochrome P450 genes
OwnerCurtis C. Harris, Harry V. Gelboin +3inventors
InventorsCurtis C. Harris, Harry V. Gelboin, Frank J. Gonzalez, Katharine C. Mace, Andrea M. A. Pfeifer
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Abstract
Non-tumorigenic, stable, human bronchial and liver epithelial cell lines are provided wherein the cell lines are capable of expressing human cytochrome P450 genes which have been inserted into the cell lines. Also provided are methods and kits for identifying potential mutagens, cytotoxins, carcinogens, chemotherapeutic and chemo-preventive agents utilizing these cell lines.
Claims11
A method for identifying or testing the mutagenicity, cytotoxicity or carcinogenicity of an agent comprising the steps of:
- a) reacting, culturing, or contacting a non-tumorigenic, stable, human bronchial epithelial cell line containing an exogenous cytochrome P450 gene, which is expressed in said cell line with an agent suspected of being a mutagen, cytotoxin, or carcinogen;
- and
- b) determining or monitoring those effects on said cell line which are indicative of mutagenicity, cytotoxicity, or carcinogenicity.
A method for identifying or testing the mutagenicity, cytotoxicity or carcinogenicity of an agent comprising the steps of:
- a) reacting, culturing, or contacting a non-tumorigenic, stable, human adult liver epithelial cell line containing an exogenous cytochrome P450 gene which is expressed in said cell line with an agent suspected of being a mutagen, cytotoxin, or carcinogen;
- and
- b) determining or monitoring those effects on said cell line which are indicative of mutagenicity, cytotoxicity, or carcinogenicity.
Dependencies are inferred from the claim text.