Cell culture media formulated in unit dose
AssigneeBio 101
InventorJames W. Gautsch
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Abstract
The present invention contemplates a system and formulations for preparing cell culture medium useful for growing cells for the purpose of producing and isolating nucleic acids. Dry-concentrate culture medium compositions are described as packaged in unit dose form such as in dissolvable capsules.
Claims18
A dry-concentrate culture medium composition in unit dose comprising an amount of cell culture medium in dry-concentrate form sufficient to prepare a preselected amount of culture medium, said dry medium packaged in unit dose form and consisting essentially of 7 grams (gms) yeast extract, 14 gms bacto-tryptone, 1.25 gms sodium chloride, 0.3 gms base and 1.6 gms-HCl per 24.15 gms total dry medium.
A dry-concentrate culture medium composition in unit dose comprising an amount of cell culture medium in dry-concentrate form sufficient to prepare a preselected amount of culture medium, said dry medium packaged in unit dose form and consisting essentially of 5 gms yeast extract, 10 gms bacto-tryptone, 10 gms sodium chloride and trace sodium hydroxide per 25 gms total dry medium, where said sodium hydroxide is present in an amount sufficient to produce a liquid medium of pH 7.0 when said dry medium is reconstituted at a ratio of 4 gms dry medium per 100 milliliters reconstituted medium.
A dry-concentrate culture medium composition in unit dose comprising an amount of cell culture medium in dry-concentrate form sufficient to prepare a preselected amount of culture medium, said dry medium packaged in unit dose form and consisting essentially of a formulation selected from the group consisting of:
- Formulation I
- 10 gms tryptone (bacto-tryptone),
- 7 gms yeast extract,
- 7 gms NaCl,
- 0.4 gms Tris Base, and
- 1.6 gms Tris HCl per 26 gms total dry medium;
- Formulation II
- 14 gms tryptone,
- 9 gms yeast extract,
- 5 gms NaCl,
- 2 gms glucose,
- 0.4 gms Tris Base, and
- 1.6 gms Tris HCl per 32 gms total dry medium;
- Formulation III
- 9gms tryptone,
- 5 gms yeast extract,
- 0.5 gms NaCl,
- 3 gms casamino acids,
- 2 gms glucose, and
- 0.5 gms Trizma pH 7-9 per 20 gms total dry medium;
- Formulation IV
- 5 gms tryptone,
- 7 gms yeast extract,
- 2.5 gms NaCl,
- 2 gms casamino acids, and
- 0.5 gms Trizma pH 7-9 per 17 gms total dry medium;
- and
- Formulation V
- 16 gms tryptone,
- 12 gms yeast extract,
- 2.5 gms NaCl,
- 2 gms MgSO4,
- 2 gms casamino acids, and
- 0.5 gms Trizma pH 7-9 per 35 gms total dry medium.
Dependencies are inferred from the claim text.