Recombinant antibodies for human therapy
AssigneeIDEC Pharmaceuticals Corporation
InventorsRoland A. Newman, Nabil Hanna, Ronald W. Raab
Life of this patent
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Abstract
Chimeric antibodies including an Old World monkey portion and a human portion, nucleic acid encoding such antibodies, Old World monkey monoclonal antibodies, and methods for their production and use.
Claims38
A chimeric antibody comprising an immunoglobulin constant region and an antigen binding region, said immunoglobulin constant region being selected from the group consisting of human immunoglobulin constant region and chimpanzee immunoglobulin constant region, and said antigen binding region being an Old World Monkey antigen-binding region.
A chimeric antibody conprising an immunoglobin variable region specific for a particular known antigen, said antibody comprising:
- (i) a constant region selected from the group consisting of human antibody constant region and chimpanzee antibody constant region;
- (ii) a framework region selected from the group consisting of human antibody framework region, chimpanzee antibody framework region and first Old World monkey antibody framework region;
- and
- (iii) a second Old World monkey antibody antigen binding portion.
A method for producing a recombinant antibody to a human antigen, said antibody being not immunogenic in a human, comprising the steps of:
- a) raising an Old World monkey antibody to said antigen in an Old World monkey,
- b) isolating an Old World monkey nucleic acid encoding an antigen-binding region of a variable region of said Old World monkey antibody,
- c) providing a human nucleic acid encoding a human constant region of a human antibody,
- d) ligating said Old World monkey nucleic acid and said human nucleic acid to form a recombinant nucleic acid, and
- e) expressing said recombinant nucleic acid to produce said recombinant antibody.
A recombinant antibody comprising
- (1) a constant region of a heavy chain of a human antibody;
- (2) a constant region of a light chain of said human antibody;
- (3) a variable region of a heavy chain of an Old World monkey antibody specific for a human antigen;
- and
- (4) a variable region of a light chain of said Old World monkey antibody.
An anti-CD4 antibody which comprises the variable light and variable heavy regions encoded by the DNA sequences set forth in SEQ ID NO:
- 20 and SEQ ID NO:
- 19.
Dependencies are inferred from the claim text.