Biosensor and method for producing the same
AssigneeMatsushita Electric Industrial Co., Ltd.
InventorsTomohiro Yamamoto, Mariko Miyahara, Toshihiko Yoshioka, Satoko Fujisawa, Shiro Nankai
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Abstract
A biosensor for rapid quantification of a specific component contained in various biological samples with high accuracy has an electrically insulating base, an electrode system including a working electrode and a counter electrode formed on one face of the insulating base, and a reaction layer formed on the insulating base in close contact with the electrode system. The reaction layer contains at least a hydrophilic polymer, a buffer and an enzyme which is separated from the buffer.
Claims18
A method for producing a biosensor comprising the steps of:
- forming a first layer containing an enzyme and a hydrophilic polymer by using water as the medium on a face of an insulating base in close contact with an electrode system including a working electrode and a counter electrode which are provided on said insulating base;
- and
- forming a second layer containing a buffer on said first layer by using an organic solvent solution of a lipid which does not dissolve said hydrophilic polymer.
A method for producing a biosensor comprising the steps of:
- forming a first layer containing a buffer and a hydrophilic polymer by using water as the medium on a face of an insulating base in close contact with an electrode system including a working electrode and a counter electrode provided on said insulating base;
- and
- forming a second layer containing a hydrophilic polymer and an enzyme on said first layer by using an organic solvent as the medium that does not dissolve said hydrophilic polymer contained in the first layer.
A method for producing a biosensor comprising:
- a first step of spreading an aqueous solution containing a hydrophilic polymer and a buffer on an insulating base in close contact with an electrode system including a working electrode and a counter electrode provided on a face of said insulating base and drying the spread solution, and
- a second step of spreading an organic solvent solution containing at least an enzyme and a hydrophilic polymer over the layer and drying the spread solution.
A method for producing a biosensor comprising the steps of:
- forming a first layer containing an enzyme and a hydrophilic polymer by using water as the medium on a face of an insulating base in close contact with an electrode system including a working electrode and a counter electrode which are provided on said insulating base;
- and
- forming a second layer containing a buffer on said first layer by using an organic solvent solution of a hydrophilic polymer, which solvent does not dissolve said hydrophilic polymer contained in the first layer.
A biosensor comprising:
- an electrical insulating base,
- an electrode system including a working electrode and a counter electrode which are provided on a face of said insulating base, and
- a reaction layer formed on said insulating base in close contact with said electrode system;
- wherein
- said reaction layer is a laminate of at least two layers, and wherein a first reaction layer is in contact with said electrode system and contains an enzyme, an electron acceptor and a hydrophilic polymer, and a second reaction layer is on top of said first reaction layer and contains a buffer and a lipid,
- said enzyme being separated from said buffer.
A biosensor comprising,
- an electrical insulating base,
- an electrode system including a working electrode and a counter electrode which are provided on a face of said insulating base, and
- a reaction layer formed on said insulating base in close contact with said electrode system;
- wherein
- said reaction layer is a laminate of at least two layers, and wherein a first reaction layer is in contact with said electrode system and contains an enzyme and an electron acceptor and a second reaction layer is on top of said first reaction layer and contains a buffer and a hydrophilic polymer, said enzyme being separated from said buffer.
A biosensor comprising,
- an electrical insulating base,
- an electrode system including a working electrode and a counter electrode which are provided on a face of said insulating base and
- a reaction layer formed on said insulating base in close contact with said electrode system;
- wherein
- said reaction layer is a laminate of at least three layers, and wherein a first reaction layer is in contact with said electrode system and contains an enzyme and an electron acceptor, a second reaction layer is on top of said first reaction layer and contains a buffer and a hydrophilic polymer and a third reaction layer is on top of said second reaction layer and contains a lipid,
- said enzyme being separated from said buffer.
Dependencies are inferred from the claim text.