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US 5,656,478 AGranted
Smooth muscle tissue formation in vivo using cultured smooth muscle cells combined with an extracellular matrix
AssigneeThe Regents of the University of California
InventorsEmil A. Tanagho, Rajvir Dahiya, Tom F. Lue, Gerald R. Cunha
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Abstract
The present invention provides a method of growing smooth muscle cells in a host with the purpose of maintaining natural cellular function by the function of smooth muscle tissue. In this method, smooth muscle cells are freed from isolated smooth muscle tissue then cultured and injected into the host in combination with an extracellular matrix.
Claims9
A method of growing smooth muscle tissue in a host, said method comprising;
- (a) isolating smooth muscle cells from a smooth muscle tissue sample;
- (b) culturing said smooth muscle cells;
- and
- (c) injecting into said host the smooth muscle cells of step (b) in combination with a solubilized basement membrane extracellular matrix comprising laminin, collagen IV, heparin sulfate proteoglycans, entactin, nidogen, and growth factors, such that natural cellular function of said injected smooth muscle cells is maintained allowing the smooth muscle cells to grow and proliferate, and smooth muscle tissue is formed.
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